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Hepatic Gene Expression Profiling of 5'AMP Induced Hypometabolism in Mice

ABSTRACT: Mice injected IP with 5'-AMP enter a hypometabolic state that is an induced hybernation (AIHM). Recovery from this AIHM is marked by reverse flip (RF) behavior Illumina arrays were used to profile control, AIHM and RF aminals in RNA prepared from the liver.

ORGANISM(S): Mus musculus

PROVIDER: GSE23975 | GEO | 2012/06/20

SECONDARY ACCESSION(S): PRJNA130461

REPOSITORIES: GEO

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Hepatic Gene Expression Profiling of 5'AMP Induced Hypometabolism in Mice

Project description:Mice injected IP with 5'-AMP enter a hypometabolic state that is an induced hybernation (AIHM). Recovery from this AIHM is marked by reverse flip (RF) behavior Illumina arrays were used to profile control, AIHM and RF aminals in RNA prepared from the liver. Animals were injected with vehicle or 0.5 mg/g body weight 5'-adenosine monophosphate. At 6 hours (AIHM) or 12 hours (RF) animals were killed and total RNA prepared from the liver.

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Project description:Homeotherms maintain a stable internal body temperature despite changing environments. During energy deficiency, some species can cease to defend their body temperature and enter a hypothermic and hypometabolic state known as torpor. Despite recent advances in our understanding of thermoregulation, the precise neurons that coordinate these profound thermoregulatory and metabolic changes remain largely unknown. Here, we demonstrate that estrogen-sensitive neurons in the medial preoptic area (MPA) are key drivers of hypothermia and hypometabolism in mice. We find that selectively activating estrogen-sensitive MPA neurons was sufficient to drive a coordinated depression of metabolic rate and body temperature similar to torpor, as measured by body temperature, physical activity, indirect calorimetry, heart rate, and brain activity. Inducing torpor with a prolonged fast revealed larger and more variable calcium transients from estrogen-sensitive MPA neurons during bouts of hypothermia. Finally, selective ablation of estrogen-sensitive MPA neurons demonstrated that these neurons are required for the full expression of fasting-induced torpor. Together, these findings suggest a role for estrogen-sensitive MPA neurons in directing the thermoregulatory and metabolic responses to energy deficiency.

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Allosteric regulation by c-di-AMP modulates global signal integration in Actinobacteria

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