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RNA-seq on primary human kidney epithelial cells with PPFIBP1 or PLEKHA1 knockdown

ABSTRACT: Here we investigated the transcriptomic changes induced by PPFIBP1 or PLEKHA1 siRNA treatments on human Primary Renal Proximal Tubule Epithelial Cells (RPTECs) with RNA-seq.

ORGANISM(S): Homo sapiens

PROVIDER: GSE240639 | GEO | 2024/02/15

REPOSITORIES: GEO

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RNA-seq on primary human kidney epithelial cells with oleate treatment and PLIN2 knockdown

Project description:Here we investigated the transcriptomic changes induced by oleic acid treatment on human Primary Renal Proximal Tubule Epithelial Cells (RPTECs) with RNA-seq. We also profiled cells with oleic acid treatment after PLIN2 gene knockdown with siRNA treatment.

2022-10-01 | GSE206084 | GEO

Tnik depletion induces inflammation and apoptosis in injured renal proximal tubule epithelial cells

Project description:We performed bulk RNA-seq on primary and immortalized human renal proximal tubule epithelial cells (RPTECs) subjected to siRNA mediated TNIK silencing.

2024-04-10 | GSE216015 | GEO

Transcription profiling of human kidney glomerular and tubular progenitor cells

Project description:Multipotent progenitor cells (MPs) have been observed in human kidneys and particularly in Bowman's capsule and proximal tubules. The kidney owns the ability to repair local damage and renal MPs may play a role in the regenerative processes. Microarray technology was applied to identify differentially expressed genes among resident MPs isolated from glomeruli and tubules of normal renal tissue, renal proximal tubular epithelial cells (RPTECs) and mesenchymal stem cells (MSCs). The results of our analysis represent a starting point for further functional studies. Experiment Overall Design: This study includes three renal tissue samples which were processed to obtain 3 glomerular progenitor populations and 3 tubular ones. Three subcoltures of MSCs and RPTECs were included as well. The differences in gene expression patterns of the 4 cell types were found out.

2009-11-09 | E-GEOD-8611 | biostudies-arrayexpress

Three-dimensional spherical cellular aggregates of RPTEC as an in vitro tool for evaluation of human renal proximal tubular toxicity and drug disposition

Project description:The proximal tubule plays an important role in the secretion and reabsorption of drugs in the kidney and is a major site of drug interaction and toxicity. Kidney toxicity analysis via in vitro assays is challenging as few provide appropriate proximal tubular cell function. In this study, we aimed to develop a simple and reproducible method to culture human proximal tubular epithelial cells (RPTECs), used in pharmacokinetic and toxicological evaluation by monitoring organic anion transporter (OAT)1 as a selection marker. As a result, by culturing RPTECs in spherical cellular aggregates, OAT1 protein expression, which does not increase in conventional 2D culture, increased over time and reached a similar level to that in human renal cortices from 5 different donors. The expression of proximal tubule markers, AQP1 and CDH6, was maintained, indicating that 3D RPTEC spheroids had proximal tubule characteristics. For SLC transporters, the 3D spheroid culture improved the protein expression of about 7% of the 139 transporter proteins detected and 2.3% of 4,800 proteins detected to about 5-fold that in human renal cortices. Furthermore, the protein expression levels of about 4800 proteins in 3D RPTEC spheroids (cultured for 12 days) were maintained over 20 days. Cisplatin and adefovir exhibited transporter-dependent ATP decreases in 3D RPTEC spheroids. These results indicate that the 3D RPTEC spheroid is a simple and robust in vitro experimental system for pharmacokinetic and toxicological evaluation in drug development.

2023-10-20 | GSE216153 | GEO

Effect of GW4064 on primary cultured mouse kidney proximal tubule cells

Project description:Global gene expression in the primary cultured mouse kidney proximal tubule cells treated either DMSO or 1uM GW4064 (a FXR agonist) was compared. Results provide insight into mechanisms underlying effects of FXR activation on gene expression in mouse kidney proximal tubule cells. Male C57/BJ mice aged 6 weeks were sacrificed under anesthesia and kidney proximal tubule cells were cultured until confluent. Cells were treated with either GW4064 (1uM) or equal amount of DMSO and incubated for 24 hours. 4 total RNA samples per group were analyzed and gene expression was compared between the groups.

2015-06-26 | E-GEOD-70296 | biostudies-arrayexpress

TGFß1-driven epithelial to mesenchymal transition in human kidney cell line

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Effect of GW4064 on primary cultured mouse kidney proximal tubule cells

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2015-06-26 | GSE70296 | GEO

Human cytomegalovirus infection triggers a paracrine senescence loop in renal epithelial cells.

Project description:We evaluated the modulation of mRNA upon human cytomegalovirus (HCMV) infection in renal proximal tubule epithelial cell line (RPTEC).

2024-02-01 | GSE185442 | GEO

TGFß1-driven epithelial to mesenchymal transition in human kidney cell line

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2011-05-30 | E-GEOD-23338 | biostudies-arrayexpress

Renal Proximal Tube Epithelial Cells at 24h and 1% oxygen

Project description:Aim of the study was to characterize the transcriptional response of human primary renal proximal tubule epithelial cells (RPTEC) to low oxygen stress.

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