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Gene expression from SCID-hu mice treated with saline (control) or intermittent PTH

ABSTRACT: The data present global gene expression profile of whole human bones, implanted in SCID mice (SCID-hu model), then engrafted with the myeloma cell line, Hg, and treated with saline or PTH for 4 weeks.

ORGANISM(S): Homo sapiens

PROVIDER: GSE24068 | GEO | 2013/09/10

SECONDARY ACCESSION(S): PRJNA130233

REPOSITORIES: GEO

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Gene expression from SCID-hu mice treated with saline (control) or intermittent PTH

Project description:The data present global gene expression profile of whole human bones, implanted in SCID mice (SCID-hu model), then engrafted with the myeloma cell line, Hg, and treated with saline or PTH for 4 weeks. SCID-hu mice were prepared by subcutaneous implantation of human bone in SCID mice. The human myeloma cell line, Hg was injected directly into the implanted bone in SCID-hum mice. Upon establishment of myeloma growth as determined by measurement of circulating human immunoglobulins in SCID-hu mice sera, the mice were treated with saline or intermittent PTH (parathyroid hormone, 80 ug/kg/day) for 4 weeks. At the end of the experiment, the human bones from SCID-hu mice were snap frozen in liquid nitrogen. RNA extracted from these bones was subjected to global gene expression profile using Affymetrix U133-Plus microarray.

2013-09-10 | E-GEOD-24068 | biostudies-arrayexpress

Expression data from placenta harvested from WT and Pth-null fetuses treated 90 minutes prior with saline or PTH (1-84)

Project description:Parathyroid hormone (PTH) plays an essential role in regulating calcium and bone homeostasis in the adult, but whether PTH is required at all for regulating fetal-placental mineral homeostasis is uncertain. To address this we treated Pth-null mice in utero with 1 nmol PTH (1-84) or saline and examined placental calcium transfer 90 minutes later. It was found that placental calcium transfer increased in Pth-null fetuses treated with PTH as compared to Pth-null fetuses treated with saline. Subsequently, to determine the effect of PTH treatment on placental gene expression, in a separate experiment, 90 minutes after the fetal injections the placentas were removed for subsequent RNA extraction and microarray analysis.

2010-03-01 | GSE16983 | GEO

Transcription profiling of mouse (SCID) after intestinal xenotransplants infected with Shigella

Project description:to analyse the transcriptomic response of human intestinal tissue engrafted in SCID mice to Shigella infection Experiment Overall Design: Four experimental conditions with two biological replicates. Experiment Overall Design: After 2 hours infection, tissues were removed and ARN were extracted before labeling and hybridizations on U-133A genechip.

2008-10-18 | E-GEOD-8636 | biostudies-arrayexpress

PTH and PTHrP treatment of primary adipocytes

Project description:Parathyroid hormone (PTH) and PTH-related protein (PTHrP) are involved in cachexia associated with chronic kidney disease and cancer respectively. Tumor-derived PTHrP triggers adipose tissue browning and thereby leads to wasting of fat tissue in tumor-bearing mice. Similarly, elevated in 5/6 nephrectomized mice, PTH stimulates adipose tissue browning and wasting. Mice lacking the PTH/PTHrP receptor in their fat tissue are resistant to wasting of both adipose tissue and skeletal muscle. Therefore, the PTH/PTHrP signaling in adipocytes should activate various pathways that contribute to hypermetabolism and muscle wasting.

2015-12-01 | GSE74082 | GEO

Mesenchymal Stem Cells (MSCs): Control vs. PTH-stimulated

Project description:Transcriptional profiling of human MSCs comparing control MSCs with parathyroid hormone (PTH)-stimulated MSCs. PTH-stimulated MSCs were treated with 0.1 nM recombinant human PTH (N-terminal fragment, amino acids 1-34) for 48 hours. Human MSCs were isolated from a bone marrow sample obtained from a healthy adult volunteer. Two-condition experiment: control MSCs vs. PTH-stimulated MSCs. 1 control MSCs and 1 PTH-stimulated MSCs.

2014-02-01 | E-GEOD-43148 | biostudies-arrayexpress

Mesenchymal Stem Cells (MSCs): Control vs. PTH-stimulated

2014-02-01 | GSE43148 | GEO

Homo sapiens

Project description:Gene expression from SCID-hu mice treated with saline (control) or intermittent PTH

| PRJNA130233 | ENA

Changes in the Bone Marrow Microenvironment Impact Anabolic Actions of PTH

Project description:Irradiation induced bone marrow ablation ultimately enhanced PTH anabolic effects in bone. B6 mice at 10 days of age were sub-lethally irradiated and treated with PTH 24h later for 5 days. 24h post last-injection, bone marrow was flushed with Trizol and RNA isolated and purified. Microarray analyses was performed to determine differential differences in PTH effects in non-irradiated vs. irradiated bone marrow. Triplicates of 4 groups (total of 12 samples) which include: Nonirradiated Vehicle, Nonirradiated PTH, Irradiated Vehicle and Irradiated PTH

2011-09-29 | E-GEOD-28819 | biostudies-arrayexpress

Kindlin-2 modulates PTH1R to regulate bone mass and PTH actions in bone

Project description:To investigation the role of PTH and Kindlin-2 in bone development, we performed single-cell RNA-sequencing. From Con-veh, Con-PTH, cKO-veh, cKO-PTH, we profiled more than 20k single cells, including multi-potent mesenchymal stromal cells (MSC), osteoprogenitors, osteoblasts, chondrocytes, fibroblasts, endothelial cells, smooth muscle cells, skeletal muscle cells, pericytes, and schwann cells. We found proportion of part of these cells were significant altered by PTH or Kindlin-2 loss, especially for MSC, osteoblast, chondrocyte, and fibroblast. Transcriptomic analysis revealed gene expression was dramatically regulated by PTH or Kindlin-2 loss.

2020-10-01 | GSE150291 | GEO

HU treatment of Sulfolobus solfataricus P2

Project description:Hydroxyurea (HU) is toxic to Sulfolobus cells. To address the basis of the HU toxicity, we performed transcriptome analyses on untreated cells and cells following exposure to 5 mM HU for 4 hours.

2016-10-05 | GSE87612 | GEO

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