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Integrated Analysis of lncRNA-miRNA-mRNA Interaction Network in Myocardial Ischemia-Reperfusion Injury During Cardiopulmonary Bypass Surgery in Mouse [RNA-seq]

ABSTRACT: The Long non-coding RNA (LncRNA) miRNA competing endogenous RNA (ceRNA) regulatory network play a pivotal role in the intricate orchestration of cardiovascular diseases, governing their onset and progression.In order to delve deeper into the ceRNA mechanism underlying myocardial ischemia-reperfusion injury (MIRI), we conducted a randomized division of six 8-week-old male C57BL/6J mice into two groups: the Sham group and the ischemia/reperfusion (I/R) group. Subsequently, we employed the Langendorff isolated heart perfusion system to carry out the experiment. Following the completion of the experiment, cardiac samples were meticulously collected and evenly partitioned into two segments: one for lncRNA library sequencing and the other for small RNA library sequencing. By leveraging the sequencing data, we meticulously scrutinized the disparities in lncRNA, miRNA, and mRNA expression profiles between the two sample sets, thereby unearthing the lncRNA-miRNA-mRNA ceRNA regulatory network that assumes a pivotal regulatory role in MIRI.

ORGANISM(S): Mus musculus

PROVIDER: GSE240701 | GEO | 2026/08/01

REPOSITORIES: GEO

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Integrated Analysis of lncRNA-miRNA-mRNA Interaction Network in Myocardial Ischemia-Reperfusion Injury During Cardiopulmonary Bypass Surgery in Mouse [miRNA-seq]

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Transcriptome analyses of cerebral ischemia reveal functional lncRNAs in ischemia stroke

Project description:Background and Purpose: Long noncoding RNAs (lncRNAs) are an emerging class of genomic regulatory molecules reported in neurodevelopment and many diseases. Despite extensive studies have identified lncRNAs and discovered their functions in CNS diseases, the function of lncRNAs in ischemia stroke remains poorly understood. Method: Ischemia was induced by transient middle cerebral artery occlusion. Expression profiles of lncRNAs, miRNAs and mRNAs after ischemia stroke were obtained using high throughput sequencing technology. A correlation network was constructed to predict lncRNA functions. LncRNA-miRNA-mRNA network was constructed to discover ceRNAs. Results: 1924 novel lncRNAs were identified, indicating that the ischemia stroke has a complex effect on lncRNAs. The top 10 regulated lncRNAs was validated by qRT-PCR. We have also predicted function of lncRNAs, and subjected them to gene co-expression network analysis, revealing the involvement of lncRNAs in many important biological process including injury and repair that are implicated in the regulation of ischemia stroke. Furthermore, lncRNAs mediated SMD (Staufen1-mediated mRNA decay) was analyzed and ceRNA (competitive endogenous RNAs) network was constructed in ischemia stroke. Conclusions: This study reports the genome-wide lncRNA profiles in ischemia stroke using high throughput sequencing and constructs a systematic lncRNA-miRNA-mRNA network which reveals a complex functional noncoding RNA regulatory network in ischemia stroke.

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Project description:Purpose：Detection of differentially expressed lncRNA in the infarct zone and the control group in myocardial ischemia-reperfusion injury model tissue. Method: Use 8 weeks of C57BL/6 mice to establish a myocardial ischemia-reperfusion injury model, 45 minutes of ischemia, and 24 hours after reperfusion, the mice were sacrificed to obtain materials. Result: The expression of lncRNAs in the infarct area of myocardial ischemia-reperfusion injury model mice was detected, and it was found that a total of 43 lncRNAs related to myocardial ischemia-reperfusion injury changed in expression, of which 17 were up-regulated (fold change >1.5). 26 expressions are down-regulated (fold change <0.8)

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MicroRNA Profiling in Ischemia-Reperfusion Injury of the Gracilis Muscle in Rats (miRNA data)

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2010-04-22 | E-GEOD-21405 | biostudies-arrayexpress

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