Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of mild respiratory SARS-CoV-2 infection on brainstem endothelial transcriptome

ABSTRACT: To investigate the transcriptional response of brainstem endothelial cells to mild respiratory infection, 12-month-old male C57Bl/6 mice were intranasally inoculated with SARS-CoV-2 MA10 strain or with saline.

ORGANISM(S): Mus musculus

PROVIDER: GSE240903 | GEO | 2023/08/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNA sequencing of lungs from WT and IFITM3 knockout mouse lungs after SARS-CoV-2 infection

Project description:WT and IFITM3 KO mice were infected with 10^5 TCID50 SARS-CoV-2 MA10 (mouse adapted virus originally generated by the Baric Lab at UNC). 2 days post infection, RNA was extracted from lung tissue using TRIzol. RNA library preparation and sequencing was performed by Genewiz.

2023-01-31 | GSE194062 | GEO

SARS-CoV-2 infection activates AhR signaling

Project description:Purpose:To determine the effect of SARS-CoV-2 infection on AhR signaling, we performed single-cell RNAseq (scRNA-Seq) from macaque lung tissue infected with SARS-CoV-2. Methods: Rhesus monkeys (3-5 kg, 3-5years old) were used for this study. One monkey is infected by intragastric administration with 1x 10^7 PFU of SARS-CoV-2 in 1mL of PBS, and another monkey was intranasally injected with 1x10^6 of SARS-CoV-2 in 200 µl of PBS. One monkey was intranasally and intragastrically treated with PBS as a control. We analyzed the tissues collected on the 4 dpi following Intranasal and intragastrical inoculation. Single-cell sequencing of were sequenced by an Illumina Novaseq6000 sequencer, the sequencing depth of each cell was at least 100,000 reads, and the paired-end 150 bp (PE150) reading strategy was adopted. Results and Conclusions: The scRNA-Seq results showed that the increased expression of upstream gene IDO1 and downstream transcriptional targets CYP1A1 and CYP1B1 of AhR in response to SARS-CoV-2 infection.

2022-08-26 | GSE211741 | GEO

Caspase-3 cleaves extracellular vesicle proteins during auditory brainstem development

Project description:Sound localization requires extremely precise development of auditory brainstem circuits, the molecular mechanisms of which are largely unknown. We previously demonstrated a novel requirement for non-apoptotic activity of the protease caspase-3 in chick auditory brainstem development. Here, we used mass spectrometry to identify proteolytic substrates of caspase-3 during chick auditory brainstem development. Functional annotation analysis revealed that our caspase-3 substrates were enriched more than two-fold for proteins associated with extracellular vesicles (EVs), membrane-bound nanoparticles that function in intercellular communication. The proteome of EVs isolated from the auditory brainstem contained caspase-3 and was highly enriched for the caspase-3 substrates identified here. Additionally, we identified two caspase-3 substrates with known functions in axon guidance, namely Neural Cell Adhesion Molecule (NCAM) and Neuronal-glial Cell Adhesion Molecule (Ng-CAM), that were found in auditory brainstem EVs and expressed in the auditory pathway alongside cleaved caspase-3. Taken together, these data suggest a novel developmental mechanism whereby caspase-3 influences auditory brainstem circuit formation through the proteolytic cleavage of EV proteins.

2020-10-16 | PXD021728 | Pride

DNA methylation alteration and gene expression sigature in brainstem, thalamic, and supratentorial gliomas

Project description:Gliomas arising in the brainstem and thalamus are devastating tumors that are difficult to surgically resect due to their proximity to eloquent brain structures. Here, we performed a comprehesive genomic and epigenomic study, using gene expression and methylation microarrays, to research on th different genomic and epigenetic signatures between brainstem, thalamic, and supratentorial gliomas. Comparison of brainstem, thalamic and supratentorial gliomas

2014-05-27 | E-GEOD-50774 | biostudies-arrayexpress

Gene Expression Analysis of Host Immune Responses in CD11b positive cells isolated from lungs of the UV inactivated SARS-CoV-immunized mice following Lethal SARS-CoV Infection

Project description:To better understand the biological pathways by which UV inactivated SARS-CoV-induced pulmonary eosinophilia occurs, we examined global transcriptional changes in macrophages from the lungs of mouse. Female BALB/c mice were used at 21 weeks of age. Mice were subcutaneously immunized with UV inactivated SARS-CoV (UV-V) or UV-V and Toll like receptor (TLR) ligands at 6 and 1 weeks prior to mouse-adapted SARS-CoV (n=6 per group). Mice were intranasally challenged with 1E+6 TCID50 in 30M-NM-<L. MEM was challenged in six mice as control infection. Mice were sacrificed and collected lungs at 1 days after challenge, then CD11b positive cells were isolated from the lungs of these mice. These cells were used for the analysis of microarray.

2014-05-22 | E-GEOD-50855 | biostudies-arrayexpress

Gene expression analysis in the Brainstem of Vglut2:Ndufs4cKO mice

Project description:Leigh Syndrome is characterized by symmetrical brain lesions and neuroinflammation in select nuclei, predominantly brainstem and/or basal ganglia. Accordingly, Ndufs4-deficient mice present overt lesions in brainstem, olfactory bulb, and basal ganglia. To define the contribution of Ndufs4 deficiency in excitatory neurons to the overall neuroinflammatory phenotype, we performed Gene expression analysis in the brainstem of mice with specific deletion of Ndufs4 in Vglut2-expressing neurons (Vglut2:Ndufs4cKO mice). This analysis allowed us to further define the inflammatory phenotype present in the brainstem of Vglut2:Ndufs4cKO mice.

2019-03-31 | GSE125470 | GEO

Single cell RNA sequencing analysis of bronchoalveolar lavage samples from SARS-CoV-2 challenged Rhesus macaques

Project description:Single cell RNA sequencing analysis was performed on bronchoalveolar lavage samples obtained from Rhesus macaques infected intranasally/intratracheally with SARS-CoV-2 after vaccination with mRNA-1273 vaccine

2022-07-18 | GSE190913 | GEO

Single-cell transcriptional mapping of the developing human brainstem

Project description:The anatomical relationship of the brainstem is complex, which is the center of respiratory circulation in the human body. We used single cell RNA sequencing (scRNA-seq) to analyze the cellular molecular diversity of the fetal brainstem.

2023-10-31 | GSE216591 | GEO

MicroRNA expression data from brainstem gliomas

Project description:Gliomas arising in the brainstem are rare tumours worldwide that are difficult to surgically resect, and the involved miRNAs and signaling pathways associated with brainstem gliomas (BSGs) are largely unclear. Tumours in the control nervous system owned a WHO classification, which are of great significance to guide the treatment and to evaluate prognosis. To determine grade-associated miRNAs in BSGs, this study performed microarray of 10 low-grade and 15 high grade BSGs.

2018-03-20 | GSE112009 | GEO