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A single-cell gene expression analysis in an in vitro co-culture model containing fibroblast cells directly mixed with NMU-manipulated lung adenocarcinoma cells

ABSTRACT: NMU gene expression was known to be over-represented in lung adenocarcinoma and negatively associated with overall survival or progression-free survival of patients. However, the mechanism(s) underlying the oncogenic roles of NMU are still not clear. Based on the transcriptome analysis of bulk-tissue of lung cancer datasets in the public domain, we found that NMU expression was significantly associated with those of some pro-fibrotic genes. Using subcutaneous mice xenograft model of human lung adenocarcinoma cells, we found significant increase of fibrotic phenotypes in xenograft derived from cell lines over-expressing NMU, including expression levels of marker proteins of myofibroblast, activation of fibroblast migration, and number of cells with morphology of fibroblast, while these changes can be reversed by knocking down the NMU expression of lung cancer cell lines. These suggest NMU from lung cancer cells may contribute to fibrosis of tumor microenvironment by recruiting and activating fibroblast and/or myofibroblast. Here we perform single-cell analysis of cell mixture containing co-cultured lung cancer cells and fibroblast cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE240911 | GEO | 2025/12/31

REPOSITORIES: GEO

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