Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE240971 | GEO | 2025/08/12

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells [RRBS]

Project description:In mammals, primordial germ cells (PGCs) undergo global erasure of DNA methylation with delayed demethylation of germline genes and selective retention of DNA methylation at young retrotransposons. However, the molecular mechanisms of persistent DNA methylation in PGCs remain unclear. Here we report that resistance to DNA methylation reprogramming in PGCs requires UHRF2, the paralog of the DNMT1 cofactor UHRF1. PGCs from Uhrf2 knock-out mice show loss of retrotransposon DNA methylation, while DNA methylation is unaffected in somatic cells of Uhrf2-/- mice. Furthermore, Uhrf2-deficient PGCs show precocious demethylation of germline genes and overexpress meiotic genes in females.

2025-08-12 | GSE240970 | GEO

UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells [RNAseq_E8.5]

2025-08-12 | GSE240968 | GEO

UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells [RNAseq_PGCs]

2025-08-12 | GSE240967 | GEO

UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells

Project description:UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells

| PRJNA1005952 | ENA

UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells [RNAseq_E8.5]

Project description:UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells [RNAseq_E8.5]

| PRJNA1005953 | ENA

UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells [RNAseq_PGCs]

Project description:UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells [RNAseq_PGCs]

| PRJNA1005954 | ENA

UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells [RRBS]

Project description:UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells [RRBS]

| PRJNA1005955 | ENA

The dynamics of genome-wide DNA methylation reprogramming in mouse primordial germ cells.

Project description:Genome-wide DNA methylation reprogramming occurs in mouse primordial germ cells (PGCs) and preimplantation embryos, but the precise dynamics and biological outcomes are largely unknown. We have carried out whole-genome bisulfite sequencing (BS-Seq) and RNA-Seq across key stages from E6.5 epiblast to E16.5 PGCs. Global loss of methylation takes place during PGC expansion and migration with evidence for passive demethylation, but sequences that carry long-term epigenetic memory (imprints, CpG islands on the X chromosome, germline-specific genes) only become demethylated upon entry of PGCs into the gonads. The transcriptional profile of PGCs is tightly controlled despite global hypomethylation, with transient expression of the pluripotency network, suggesting that reprogramming and pluripotency are inextricably linked. Our results provide a framework for the understanding of the epigenetic ground state of pluripotency in the germline.

| S-EPMC3533687 | biostudies-literature

Comparative Principles of DNA Methylation Reprogramming during Human and Mouse In Vitro Primordial Germ Cell Specification.

Project description:Primordial germ cell (PGC) development is characterized by global epigenetic remodeling, which resets genomic potential and establishes an epigenetic ground state. Here we recapitulate PGC specification in vitro from naive embryonic stem cells and characterize the early events of epigenetic reprogramming during the formation of the human and mouse germline. Following rapid de novo DNA methylation during priming to epiblast-like cells, methylation is globally erased in PGC-like cells. Repressive chromatin marks (H3K9me2/3) and transposable elements are enriched at demethylation-resistant regions, while active chromatin marks (H3K4me3 or H3K27ac) are more prominent at regions that demethylate faster. The dynamics of specification and epigenetic reprogramming show species-specific differences, in particular markedly slower reprogramming kinetics in the human germline. Differences in developmental kinetics may be explained by differential regulation of epigenetic modifiers. Our work establishes a robust and faithful experimental system of the early events of epigenetic reprogramming and regulation in the germline.

| S-EPMC5064768 | biostudies-literature

Comparative principles of DNA methylation reprogramming during human and mouse in vitro primordial germ cell specification

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-10-10 | GSE86586 | GEO