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Control of poly(A)-tail length and translation in vertebrate oocytes and early embryos

ABSTRACT: During oocyte maturation and early embryonic development, poly(A)-tail lengths strongly influence mRNA translation. However, how tail lengths are controlled at different developmental stages has been unclear. Here, we performed tail-length and translational profiling of mRNA reporter libraries (each with > 10 million 3ʹ-UTR sequence variants) in frog oocytes and embryos, and fish embryos. These analyses revealed that the UUUUA motif specifies cytoplasmic polyadenylation and identified diverse context features that modulate the activity of this 5-mer. Additional sequence motifs drive stage-specific deadenylation in embryos, and UUUUA and C-rich motifs drive tail-length-independent translational repression in oocytes. A neural network model accurately predicts tail-length change during oocyte maturation in frogs, mice, and humans. Analyses of human sequence variants showed that those predicted to disrupt tail-length control have been under negative selection, implying that our insights into control of poly(A)-tail length and translation have implications for human health and fertility.

ORGANISM(S): Mus musculus Xenopus laevis synthetic construct Danio rerio

PROVIDER: GSE241107 | GEO | 2024/02/15

REPOSITORIES: GEO

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mRNA Poly(A)-tail Changes Specified by Deadenylation Broadly Reshape Translation in Drosophila Oocytes and Early Embryos

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2016-08-08 | GSE83616 | GEO

mRNA Poly(A)-tail Changes Specified by Deadenylation Broadly Reshape Translation in Drosophila Oocytes and Early Embryos

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2016-08-08 | E-GEOD-83616 | biostudies-arrayexpress

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2014-01-29 | GSE52809 | GEO

Poly(A)-tail profiling reveals an embryonic switch in translational control

2014-01-29 | E-GEOD-52809 | biostudies-arrayexpress

mTAIL-seq reveals dynamic poly(A) tail regulation in oocyte-to-embryo development [RNA-seq Drosophila oocyte stages]

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2016-08-01 | E-GEOD-83730 | biostudies-arrayexpress

Ultrasensitive Ribo-seq reveals translational landscapes during mammalian oocyte-to-embryo transition and pre-implantation development

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