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Single-cell RNA sequencing of ST2 mouse cell line

ABSTRACT: Single-cell transcription was studied in the stromal bone marrow derived cell line ST2.

ORGANISM(S): Mus musculus

PROVIDER: GSE241567 | GEO | 2025/08/08

REPOSITORIES: GEO

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GRO-seq profiles in ST2 cell line

Project description:Nascent transcription was studied using GRO-seq in the stromal bone marrow derived cell line ST2.

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Gene expression of murine long-lived bone marrow plasma cells ex vivo and plasma cells cultured for 3 days with ST2 stromal cells and the cytokine APRIL under physiological oxygen conditions.

Project description:We compared gene expression profiles of murine longlived bone marrow plasma cells isolated from immunized C57BL/6J in the memory phase with plasma cells co-cultured for 3 days with murine ST2 stromal cells in the presence of the cytokine APRIL under physiological oxygen conditions.

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Project description:To investigate the effect of interleukin (IL-33) on bone marrow (BM) resident type 2 innate lymphoid cells (ILC2s), we treated BL/6 and ST2-knockout (ST2-/-) ILC2s for 24h with recombinant mouse IL-33 We then performed gene expression profiling analysis using data obtained from RNA-seq of 3-4 samples per condition.

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Project description:Foxp3+ regulatory T cells (Tregs) are critical mediators of peripheral tolerance and immune homeostasis. Tregs that express the IL-33 receptor ST2 are enriched in peripheral nonlymphoid tissues and can exert a variety of tissue-specific functions from metabolic regulation within adipose tissue to skeletal muscle repair. However, the relationship between ST2+ and ST2- Tregs within and across different tissues remains unclear. To compare murine ST2- and ST2+ Tregs within and across tissues, we performed RNA sequencing (RNAseq) of ST2-CD44hi and ST2+CD44hi Tregs from blood, spleen, lungs, visceral adipose tissue (VAT), colon, and skin. RNAseq was also performed on ST2- CD44lo CD62L+ Tregs from the spleen and lungs. We found that the tissue microenvironment was the major factor shaping the transcriptome of Tregs across tissues. Across the tissues studied, Treg transcriptomes displayed an ordered hierarchy that may represent graded levels of activation or differentiation across tissues. We also identified a core signature that distinguished ST2+ Tregs from ST2- Tregs across tissues and a large number of differentially expressed genes between ST2- and ST2+ Tregs within individual tissues that could support the tissue-specific adaptation and function of ST2+ Tregs. In summary, our work highlights the unique, tissue-specific phenotype of ST2+ Tregs and reveals a core ST2+ Treg transcriptional signature shared across tissues.

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