S-phase-induced RNAs licensing DNA replication control ORC1 engagement at early DNA origin sites [RNA-seq]

ABSTRACT: Coordinated initiation of DNA replication is essential to ensure efficient and timely DNA synthesis. Yet the mechanism governing the “initiation” process in eukaryotic cells remains elusive. Here, we present data demonstrating a novel feature of RNAs transcribed in the proximity of actively replicating gene loci. We show that S-phAse-RNAs aNChoring ORC1 (ANCORs) to the histone variant H2A.Z are licensors of the DNA replication process. The concomitant ANCOR-H2A.Z interaction is essential for the cell to initiate duplication of their genetic heritage. Widespread and locus-specific perturbations of these transcripts correlate with anomalous replication patterns and loss of the replicative marker at the origin site.

ORGANISM(S): Homo sapiens

PROVIDER: GSE241680 | GEO | 2026/08/01

REPOSITORIES: GEO