Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Carbon starvation and pectin utilization in Aspergillus nidulans

ABSTRACT: We have a cDNA microarray to investigate changes in gene expression following transfer of fungal cultures from growth on glucose to growth on pectin or no carbon source. Our goal was to asses the roles of release from carbon catabolite repression and specific induction on proteins needed for metabolism (or utilization) of a single class of complex polysaccharide. Keywords = Aspergillus Keywords = Pectin Keywords = central metabolism Keywords = pectin Keywords = carbon catabolite repression Keywords = polysaccharide Keywords = exopolygalacturonase Keywords: time-course

ORGANISM(S): Aspergillus nidulans

PROVIDER: GSE2417 | GEO | 2006/01/01

SECONDARY ACCESSION(S): PRJNA91127

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Carbon starvation and pectin utilization in Aspergillus nidulans

Project description:We have a cDNA microarray to investigate changes in gene expression following transfer of fungal cultures from growth on glucose to growth on pectin or no carbon source. Our goal was to asses the roles of release from carbon catabolite repression and specific induction on proteins needed for metabolism (or utilization) of a single class of complex polysaccharide. Keywords = Aspergillus Keywords = Pectin Keywords = central metabolism Keywords = pectin Keywords = carbon catabolite repression Keywords = polysaccharide Keywords = exopolygalacturonase

2010-06-11 | E-GEOD-2417 | biostudies-arrayexpress

Regulation of nitrogen and carbon metabolism in Aspergillus nidulans.

Project description:In Aspergillus nidulans, nitrogen and carbon metabolism are under the control of wide-domain regulatory systems, including nitrogen metabolite repression, carbon catabolite repression. Transcriptomic analysis of the wild type strain grown under different combinations of carbon and nitrogen regimes was performed, to identify differentially regulated genes. Carbon metabolism predominates as the most important regulatory signal but for many genes, both carbon and nitrogen metabolisms coordinate regulation.

2019-05-15 | GSE115021 | GEO

Widespread glucose-mediated repression of plant biomass utilization in the white-rot fungus Dichomitus squalens

Project description:Carbon catabolite repression (CCR) occurs in the presence of sufficient concentrations of easy metabolizable carbon sources (e.g. glucose), down-regulating the expression of genes encoding enzymes involved in the breakdown of complex carbon sources. The extent of CCR at a global level is unknown in wood-rotting fungi – and was investigated in Dichomitus squalens with transcriptomics and exo-proteomics. Approximately 10% of expressed genes had lower expression in presence of glucose compared to Avicel or xylan alone. The glucose repressed genes included key components for utilization of plant biomass – CAZymes, sugar transporters and carbon catabolic genes. The majority of polysaccharide degrading CAZymes were repressed and included activities towards all major polymers. The repression found in the transcriptome was strongly supported by exo-proteomics – there was repression of almost all of the CAZymes whose transcripts were repressed on Avicel. The clear CCR-mediated effects indicate biotech production of CAZymes by D. squalens would benefit from de-repressed or constitutively expressing strains. The extent of CCR is surprising given that D. squalens rarely experiences high sugar concentrations in its woody environment.

2019-10-07 | PXD014774 | Pride

Transcriptomic analysis of Alishewanella species utilizing pectin as a sole carbon source

Project description:Comparative transcriptomic analysis of three Alishewanella species utilizing pectin as a sole carbon source

2013-03-29 | GSE45511 | GEO

Transcriptomic analysis of Alishewanella species utilizing pectin as a sole carbon source

Project description:Comparative transcriptomic analysis of three Alishewanella species utilizing pectin as a sole carbon source Gene expression level of three Alishewanella species grown on pectin was investigated and compared with A. jeotgali grown on succinate

2013-03-29 | E-GEOD-45511 | biostudies-arrayexpress

RNA-seq analysis of Bacteroides xylanisolvens XB1AT grown on pectin or xylan

Project description:Dietary fiber degradation is a key function of the human gut microbiota. The aim of this study was to increase our knowledge on the degradation of plant cell wall polysaccharide degradation by a prominent human gut bacterial species, Bacteroides xylanisolvens. The transcriptome analysis of B. xylanisolvens XB1AT revealed the existence of six and two genomic loci dedicated to the degradation of pectins and xylan, respectively. These loci or PUL ("Polysaccharide Utilization Loci") are known to encode enzyme systems in Bacteroides that are specific to a particular polysaccharide. Simple two-way comparisons between pectin or xylan sources (treatment) and glucose or xylose (control), collected during mid- and late-log phase. Three replicates per condition.

2015-10-28 | E-GEOD-74379 | biostudies-arrayexpress

Transcription profiling of Aspergillus niger grown in media containg different pectic substrates to study the pectinolytic system

Project description:To study the induction of the genes encoding known and putative enzymes from the pectinolytic system of A. niger, the transcriptional profiles of 58 selected known or putative pectinolytic genes were monitored by microarray experiments. For this purpose, A. niger was cultivated on the complex substrates, sugar beet pectin and polygalacturonic acid as primary carbon sources. Galacturonic acid, rhamnose and xylose were used to assess the effects on gene expression caused by simple well-defined carbon sources, representing the most abundant sugar residues present in the backbone of pectin. Fructose, as a strong repressor of the expression of genes that are under carbon catabolite regulation, and sorbitol, as a non-inducing sugar-like alcohol, which does not affect the carbon catabolite regulation mechanisms were selected as control substrates. Mycelia of A. niger were pregrown for 18 h on 2% fructose, transferred to medium containing the different pectic and control substrates, and sampled at four time points during 24 h of incubation.

2008-06-13 | E-MEXP-1626 | biostudies-arrayexpress

Fish oil/pectin temporally alters gene expression profiles in exfoliated rat colonocytes throughout oncogenesis

Project description:We have demonstrated that fish oil/pectin (FO/P) diets protect against colon cancer compared to corn oil/cellulose (CO/C) by upregulating apoptosis and suppressing proliferation. To elucidate the mechanisms whereby FO/P diets induce apoptosis and suppress proliferation during the tumorigenic process, we analyzed the temporal gene expression profiles from exfoliated rat colonocytes. KEYWORDS: Fish oil/pectin, pectin, exfoliated colonocytes, gene expression, apoptosis, colon cancer, chemoprevention

2012-02-20 | GSE27266 | GEO

Yung_npj_Biofilms_Microbiome_2019_ProteomicsData

Project description:Comparison of Pseudomonas aeruginosa planktonic cultures and bacterial biofilms grown under variations in growth media to demonstrate carbon catabolite repression phenomena using bottom-up proteomics

2019-08-13 | MSV000084204 | MassIVE