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Comparative expression analysis between TTHA0101-deficient mutant and wild-type of Thermus thermophilus HB8

ABSTRACT: We observed the expression profile of the total mRNA of TTHA0101 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70°C compared with that of wild type.

ORGANISM(S): Thermus thermophilus HB8

PROVIDER: GSE24184 | GEO | 2010/10/15

SECONDARY ACCESSION(S): PRJNA130111

REPOSITORIES: GEO

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Comparative expression analysis between TTHA0101-deficient mutant and wild-type of Thermus thermophilus HB8

Project description:We observed the expression profile of the total mRNA of TTHA0101 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70Â°C compared with that of wild type. Four wild-type and three mutant T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 680 min, and then crude RNA was extracted. In order to compare mRNA expression in the TTHA0101 deletion mutant with that of wild type, the expression of each mRNA was analyzed on a GeneChip.

2010-10-15 | E-GEOD-24184 | biostudies-arrayexpress

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