Targeting oncogene-dependent TIM-3 induces T cell reprogramming thereby enhancing graft-versus-leukemia immunity - scRNAseq

ABSTRACT: We use a mouse model for AML treatment in mice, based on injection of WEHI-3B AML cells followed by allogeneic transplantation of bone marrow and T cells. Allogeneic donor T cells will elicit anti-leukemic GVL effect. We want to decipher the importance of TIM-3 expression on T cells, therefore we used donor BM and donor T cells that contains a deletion for TIM-3 in CD8+ T cells (Havcr2fl/fl;E8icre/+). We performed single cell RNA sequencing and compared T cells isolated from Havcr2fl/fl;E8icre/+ or Havcr2fl/fl (control).

ORGANISM(S): Mus musculus

PROVIDER: GSE242334 | GEO | 2024/07/24

REPOSITORIES: GEO