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Salicylic acid and RNA interference mediate antiviral immunity of plant stem cells (Previous preprint title: A hormone-activated mobile RNAi pathway defends plant stem cells from virus infection)

ABSTRACT: Study on the role of Arabidopsis thaliana RDR1 and other RNAi factors in the exclusion of RNA viruses from the shoot apical meristem stem cells

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE242499 | GEO | 2023/10/09

REPOSITORIES: GEO

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A hormone-activated mobile RNAi pathway defends plant stem cells from virus infection

Project description:Study on the role of Arabidopsis thaliana RDR1 and other RNAi factors in the exclusion of RNA viruses from the shoot apical meristem stem cells

2023-10-09 | GSE221157 | GEO

A plant immune protein enables broad antitumor response by rescuing microRNA deficiency in cancers [mRNA-seq]

Project description:Cancer cells are featured with uncontrollable activation of cell cycle and proliferation,and microRNA deficiency drives tumorigenesis. The RNA-dependent RNA polymerase (RDR) is essential for small RNA-mediated immune response in plants, but absent in vertebrates with adaptive immunity. Here we show that ectopic expression of plant RDR1 can generally inhibit the proliferation of different cancer cells. Interestingly, we find that abnormal AGO2-free microRNA isoforms with 1-nucleotide shortening at the 3’ end are widely accumulated in many tumors. RDR1 with nucleotidyltransferase activity can recognize and modify these problematic microRNAs with mononucleotides to restore their AGO2 loading efficiency, which eventually rescues microRNA deficiency and elevates microRNA expression to target cell cycle in cancer specifically. The wide antitumor effects of RDR1 can be visualized in multiple xenograft tumor models in vivo, which can be realized by adeno-associated virus-mediated delivery. Altogether, we develop a broad microRNA-mediated antitumor stratagem using the plant immune protein RDR1.

2022-03-08 | GSE198145 | GEO

A plant immune protein enables broad antitumor response by rescuing microRNA deficiency in cancers [miRNA-seq]

2022-03-08 | GSE198146 | GEO

A plant immune protein enables broad antitumor response by rescuing microRNA deficiency in cancers [RNA-seq and smallRNA-seq]

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Deep sequencing of uncapped RNA in rdr1/2/6 and wild-type Arabidopsis plants

Project description:Our previous experiment on biogenesis mechanisim of stress-inducible antisense RNAs showed that accumulation of antisense RNA in a drought-inducible gene, RD29A locus was decreased in rdr1/2/6 triple mutant. The RD29A antisense RNA did not have CAP structure and poly(A) tail. We applied deep sequencing of uncapped RNA using SOLiD to identify RDR1/2/6-mediated antisense RNAs in genome-wide. We identified 1) 975 RDR1/2/6-mediated antisense RNA loci on which no. of sequence tags is decreased in rdr1/2/6 and makes peak and 2) 1,613 RDR1/2/6-mediated antisense RNA loci on which no. of the tags is decreased in rdr1/2/6 and enriched on antisense strand of exons. They were grouped into 1,959 non-redundant loci.

2017-07-13 | GSE39033 | GEO

Plant Screen Mobile: an open-source mobile device app for plant trait analysis.

Project description: Not available

| S-EPMC6329080 | biostudies-literature

Indentification samll RNA loci that are synthesized by RNA-dependent RNA polymerases1/2/6

Project description:To identify RDR1/2/6 dependent small RNA loci of transcribed regions under 2hr drought stress- and untreated conditions, expression profiles of small RNA between rdr1/2/6 and wild-type using hi-seq 2000. rdr1/2/6 and wild-type plants (Arabidopsis thaliana ecotype Columbia) were grown on MS medium for 2weeks. Then, their plants were transfered to dry plastic dish for 2 hours. small RNA fraction was s extracted from the sample using Ambion mirVana miRNA Isolation Kit. small RNA labrary was constructed using TruSeq Small RNA Library Preparation Kits and applied to hi-seq 2000.

2016-03-31 | E-GEOD-72982 | biostudies-arrayexpress

Biological function of antisense RNAs that are synthesized by RNA-dependent RNA polymerases under rehydration

Project description:To identify RDR1/2/6-dependent antisense RNA loci under 2hr drought stress and 2hr rehydration, expression profiles of Poly (A+) RNA between rdr1/2/6 and wild-type using the custom microarray (GPL19830) were analyzed. rdr1/2/6 and wild-type plants (Arabidopsis thaliana ecotype Columbia) were grown on MS medium for 2 weeks. Then, the plants were transferred to plastic dish and kept for 2hr. Poly (A+) RNA from 100Î¼g total RNA by treating with Ambion Poly A purist twice.5 ng of Poly (A+) RNA were used for the expresion profiling using the custom array (GPL19830).

2016-07-31 | E-GEOD-83846 | biostudies-arrayexpress

Salicylic acid activates DNA damage responses to potentiate plant immunity.

Project description: Not available

| S-EPMC3863363 | biostudies-literature

Ghosts in the Machine II: Neural Correlates of Memory Interference from the Previous Trial.

Project description: Not available

| S-EPMC6059123 | biostudies-literature