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Expression of transcriptional factors in age-associated B cells

ABSTRACT: To gain insights into the nature of human age-associated B cells (ABCs), we sorted peripheral B cells from a patient with new-onset SLE and performed droplet-based scRNA-seq. Seven distinct clusters were revealed by unsupervised clustering with a two-dimensional uniform manifold approximation and projection (UMAP). These clusters were assigned to known peripheral B cell subsets including transitional B cells, naïve B cells, activated naïve B cells, ABCs, memory B cells, plasmablasts, and plasma cells by comparing differentially expressed genes with established landmark genes. ABCs preferentially expressed genes encoding key surface markers CD19, CD86, FCRLA, FCRL3/5, FCGR2B, MS4A1 and ITGAX. We found 43 differentially expressed TFs: 27 upregulated and 16 downregulated.

ORGANISM(S): Homo sapiens

PROVIDER: GSE242615 | GEO | 2023/12/31

REPOSITORIES: GEO

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Transcriptomic analysis of human B cell responses to vaccination

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