Genomics

Dataset Information

0

The Drosophila melanogaster Transcriptome by Paired-End RNA-Sequencing


ABSTRACT: RNA-seq was used to generate an extensive map of the Drosophila melanogaster transcriptome by broad sampling of 10 developmental stages. In total, 142.2 million uniquely mapped 64-100bp paired-end reads were generated on the Illumina GA II yielding 356x sequencing coverage. More than 95% of FlyBase genes and 90% of splicing junctions were observed. Modifications to 30% of FlyBase gene models were made by extension of untranslated regions, inclusion of novel exons, and identification of novel splicing events. A total of 319 novel transcripts were identified, representing a 2% increase over the current annotation. Alternate splicing was observed in 31% of D. melanogaster genes, a 38% increase over previous estimations, but significantly less than that observed in higher organisms. Much of this splicing is subtle such as tandem alternate splice sites.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE24324 | GEO | 2010/09/24

SECONDARY ACCESSION(S): PRJNA132851

REPOSITORIES: GEO

Similar Datasets

| E-GEOD-24324 | biostudies-arrayexpress
| E-GEOD-40524 | biostudies-arrayexpress
2019-01-01 | GSE120387 | GEO
2013-07-30 | GSE40524 | GEO
2023-01-26 | GSE102537 | GEO
| E-GEOD-22588 | biostudies-arrayexpress
2014-06-11 | GSE58379 | GEO
2010-07-20 | GSE22588 | GEO
| E-GEOD-43223 | biostudies-arrayexpress
2017-06-01 | GSE83923 | GEO