Nanostring of gene expression in intrahepatic leukocytes from mouse fed a chow or high-fat, -fructose, and -cholesterol (FFC) diet and treated with vehicle or a pharmacological inhibitor of the receptor for advanced glycation end products (RAGE), TTP488
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ABSTRACT: Intrahepatic macrophages in nonalcoholic steatohepatitis (NASH) are heterogenous and include proinflammatory recruited monocyte derived macrophages. RAGE is expressed on macrophages and can be activated by damage associated molecular patterns (DAMPs) upregulated in NASH, yet the role of macrophage-specific RAGE signaling in NASH is unclear. Therefore, we hypothesized that a subset of RAGE expressing macrophages are proinflammatory and mediate liver inflammation in NASH. We profiled the transcriptome of intrahepatic leukocytes in a murine model of diet induced NASH where mice received vehicle or a pharmacological RAGE inhibitor (TTP488). By Nanostring nCounter Immunology Codeset Analysis of IHLs, we observed that RAGE inhibition resulted in amelioration of FFC diet induced activation of macrophage and T cell pathways which was associated with attenuation of liver inflammation.
ORGANISM(S): Mus musculus
PROVIDER: GSE243293 | GEO | 2023/09/15
REPOSITORIES: GEO
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