Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of Gene Products Involved in the Oxidative Stress Response of Moraxella catarrhalis: Evaluation of OxyR dependent Genes

ABSTRACT: Series of DNA microarrays (4) comparing the M. catarrhalis strain 035E and M. catarrhalis oxyR mutant response to 50 mM hydrogen peroxide.

ORGANISM(S): Moraxella catarrhalis

PROVIDER: GSE24351 | GEO | 2010/12/07

SECONDARY ACCESSION(S): PRJNA132801

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Identification of Gene Products Involved in the Oxidative Stress Response of Moraxella catarrhalis: Evaluation of OxyR dependent Genes

Project description:Series of DNA microarrays (4) comparing the M. catarrhalis strain 035E and M. catarrhalis oxyR mutant response to 50 mM hydrogen peroxide. Wild-type cells and oxyR mutant cells were exposed to 50 mM hydrogen peroxide for 15 minutes. RNA was extracted and DNA microarray analysis performed. 4 biologic replicates were studied. One dye swap was included in this series analysis. Control oxyR mutant cells exposed to water

2010-12-07 | E-GEOD-24351 | biostudies-arrayexpress

Identification of Gene Products Involved in the Oxidative Stress Response of Moraxella catarrhalis

Project description:Series of DNA microarrays (4) comparing the M. catarrhalis strain 035E response to 50 mM hydrogen peroxide relative to a water-only control.

2010-12-07 | GSE24331 | GEO

Identification of Gene Products Involved in the Oxidative Stress Response of Moraxella catarrhalis

Project description:Series of DNA microarrays (4) comparing the M. catarrhalis strain 035E response to 50 mM hydrogen peroxide relative to a water-only control. Wild-type cells were exposed to either 50 mM hydrogen peroxide or a water-only control for 15 minutes. RNA was extracted and DNA microarray analysis performed. 4 biologic replicates were studied. One dye swap was included in this series analysis.

2010-12-07 | E-GEOD-24331 | biostudies-arrayexpress

Transcriptional response of Acinetobacter baumannii to hydrogen peroxide and role of mumR and oxyR in regulating this response

Project description:Acinetobacter baumannii is a Gram-negative opportunistic pathogen that causes multiple infections, including pneumonia, bacteremia, and wound infections. Due to multiple intrinsic and acquired drug-resistance mechanisms, A. baumannii isolates are commonly multi-drug resistant and infections are notoriously difficult to treat. Therefore, it is important to identify mechanisms used by A. baumannii to survive stresses encountered during infection as a means of identifying new drug targets. In this study, we determined the transcriptional response of A. baumannii to hydrogen peroxide stress using RNASequencing. Upon exposure to hydrogen peroxide, A. baumannii differentially transcribes several hundred genes. In this study, we also determined the transcriptional profile of A. baumannii strains with the transcriptional regulators mumR or oxyR genetically inactivated and identified transcriptional differences between these strains and wild-type A. baumannii in response to hydrogen peroxide stress. In doing this, the function of A. baumannii OxyR in hydrogen peroxide stress resistance and regulation of genes required for hydrogen peroxide detoxification was defined. Moreover, the contribution of the uncharacterized regulator MumR to hydrogen peroxide stress resistance was also explored. This work reveals the transcriptome of an important human pathogen in the presence of hydrogen peroxide stress.

2018-11-13 | GSE114130 | GEO

Bacteroides fragilis oxidative stress

Project description:The idea was to examine the role of OxyR in control of the oxidative stress response of B. fragilis when exposed to either atmospheric oxygen, 5% oxygen atmosphere, or hydrogen peroxide Keywords: stress response

2007-12-30 | GSE4583 | GEO

Moraxella catarrhalis

Project description:Identification of Gene Products Involved in the Oxidative Stress Response of Moraxella catarrhalis: Evaluation of OxyR dependent Genes

| PRJNA132801 | ENA

Characterization of the oxyR mutant in the intracellular pathogen Legionella pneumophila

Project description:The intracellular pathogen Legionella pneumophila (Lp) is a strict aerobe, surviving and replicating in environments where it frequently encounters reactive oxygen species, such as the nutrient-poor water environment and inside host cells. In many proteobacteria, the oxidative stress response is regulated by the LysR-type regulator OxyR; however, the role played by the OxyR homologue in Lp is still unclear. Therefore, we undertook the characterisation of the phenotypes associated with the deletion of OxyR in Lp. OxyR is dispensable for growth in rich broth, in amoeba and in human cultured macrophages, and for the survival of Lp in water. Nevertheless, the mutant was found to be more sensitive to hydrogen peroxide than the wild-type when grown to post-exponential phase, but not when grown to exponential phase. Moreover, the mutant is defective in forming isolated colonies on charcoal yeast extract (CYE) agar plates, but supplementation with anti-ROS molecules, such as pyruvate, α-ketoglutarate and catalase, rescued this defect. Further characterisation of this phenotype using a transcriptional reporter fusion and microarray analysis revealed that the deletion mutant is not defective for the expression of known anti-ROS genes which suggests that the growth defect on agar plates and the higher susceptibility to hydrogen peroxide are due to a broad change in the transcriptional response. Furthermore, the growth defect is suppressed when the mutant is grown on CYE plates made with agarose, suggesting that a compound present in typical agar is toxic for the oxyR mutant.

2021-07-20 | GSE87029 | GEO

Deciphering the genetic basis of Moraxella catarrhalis complement resistance: Gene expression analysis of Moraxella catarrhalis BBH18 upon exposure to normal human serum.

Project description:The complement system is an important part of the innate defense against invading pathogens. The ability to resist complement-mediated killing is considered to be an important virulence trait for the human-restricted respiratory tract pathogen M. catarrhalis, as most disease-associated M. catarrhalis isolates are complement-resistant. Here we studied the molecular basis of M. catarrhalis complement-resistance by transcriptome profiling upon exposure to 10% normal human serum (NHS).

2014-06-23 | GSE52552 | GEO

Analysis of the Zur Regulon of Moraxella catarrhalis

Project description:This series of microarrays explores the genes regulated by the Zur gene of Moraxella catarrhalis.

2014-03-23 | GSE28151 | GEO

Moraxella catarrhalis

Project description:Moraxella catarrhalis Genome sequencing

| PRJNA688249 | ENA