Mouse models to investigate in situ cell fate decisions induced by TP53 and other factors (CUT&RUN)

ABSTRACT: We developed triple-FLAG TRP53 knock-in mice for TRP53 pull-down and two TRP53 response reporter mice, knocking tdTomato or GFP into the Puma/Bbc3 or p21 gene loci, respectively. By crossing the reporter mice onto a TRP53-deficient background, we could show that these reporters reliably inform on TRP53-dependent and TRP53-independent initiation of the apoptotic as well as the cell cycle arrest/senescence programs in vitro and even in vivo.

ORGANISM(S): Mus musculus

PROVIDER: GSE243999 | GEO | 2024/06/28

REPOSITORIES: GEO