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Slide-tags: scalable, single-nucleus barcoding for multi-modal spatial genomics

ABSTRACT: Recent technological innovations have enabled the high-throughput quantification of gene expression and epigenetic regulation within individual cells, transforming our understanding of how complex tissues are constructed. Missing from these measurements, however, is the ability to routinely and easily spatially localise these profiled cells. We developed a strategy, Slide-tags, in which single nuclei within an intact tissue section are ‘tagged’ with spatial barcode oligonucleotides derived from DNA-barcoded beads with known positions. These tagged nuclei can then be used as input into a wide variety of single-nucleus profiling assays. We used Slide-tags to profile two different stages of development in the mouse brain.

ORGANISM(S): Mus musculus

PROVIDER: GSE244355 | GEO | 2023/10/03

REPOSITORIES: GEO

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