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Temporal transcriptomic analysis of the Arabidopsis thaliana - Botrytis cinerea interaction

ABSTRACT: Transcriptional profiling of Arabidopsis leaves comparing mock-treated leaves with Botrytis cinerea infected leaves over a time-course (12 and 24 hrs).

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE24445 | GEO | 2011/03/01

SECONDARY ACCESSION(S): PRJNA132685

REPOSITORIES: GEO

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Temporal transcriptomic analysis of the Arabidopsis thaliana - Botrytis cinerea interaction

Project description:Transcriptional profiling of Arabidopsis leaves comparing mock-treated leaves with Botrytis cinerea infected leaves over a time-course (12 and 24 hrs). Two-condition experiment, Mock-treated vs infected leaves. Biological replicates: 5 Mock-treated, 5 infected, independently grown and harvested. One replicate per array.

2011-03-01 | E-GEOD-24445 | biostudies-arrayexpress

Whole Genome Bisulfite Sequencing of Botrytis cinerea

Project description:Analysis of the genome-wide DNA methylation pattern of Botrytis cinerea. Results provide new and important information that DNA methylation is critical for pathogenicity and development of Botrytis cinerea by regulating gene expression.

2019-10-01 | GSE114821 | GEO

Whole Genome Bisulfite Sequencing Data of Botrytis cinerea

2021-06-01 | GSE131718 | GEO

Expression data of sound vibration-treated Arabidopsis thaliana during Botrytis cinerea infection

Project description:Sound vibration (SV), a mechanical stimulus, can trigger various molecular and physiological changes in plants. Herein, we investigated the effect of SV pre-treatment on Arabidopsis immunity to measure the priming potential of SV. Arabidopsis plants (fourteen-day-old) were treated with sound vibration (1000 Hz, 100 dB) for daily 3 hours up to 10 days in a soundproof chamber. The control plants were kept in a similar sound-proof chamber without SV exposure (daily 3 h) up to 10 days. After that, control and SV-treated plants were challenged with Botrytis cinerea spores. The result showed that SV pre-treatment increases the disease resistance of Arabidopsis against B. cinerea. Samples from three different time points were analyzed through microarray: (1) right after the 10th day of 3h SV treatment (0 h time point), and (2) after Botrytis spore inoculation (12 and 24 hpi time points). RNA was isolated from rosette leaves.

2017-04-29 | E-MTAB-4077 | biostudies-arrayexpress

Arabidopsis defense against Botrytis cinerea: chronology and regulation deciphered by high-resolution temporal transcriptomic analysis

Project description:This SuperSeries is composed of the following subset Series: GSE29642: Arabidopsis defense against Botrytis cinerea: chronology and regulation deciphered by high-resolution temporal transcriptomic analysis (time series) GSE39597: Arabidopsis defense against Botrytis cinerea: chronology and regulation deciphered by high-resolution temporal transcriptomic analysis (tga3-2 knockout data) Refer to individual Series

2012-10-03 | E-GEOD-39598 | biostudies-arrayexpress

Spatial transcriptomic analysis of the Arabidopsis thaliana - Botrytis cinerea interaction

Project description:A 10 ul drop of B. cinerea spores prepared in half-strength commercial grape juice was placed in the middle of detached Arabidopsis leaves. A cock-borer of 6 mm diameter was used to harvest leaf discs starting from the edge of the lesion (close, 0-6 mm) and the edge of the first disc (away, 6-12 mm) 48 hr after inoculation. Control plants were inoculated with a 10 ul drop of half-strength commercial grape juice with no spores.

2011-03-01 | GSE24444 | GEO

Botrytis cinerea

Project description:Botrytis cinerea infection on Solanum lycopersicum leaves Raw sequence reads

| PRJNA496584 | ENA

Transcription profiling of tomato fruit responses to Botrytis cinerea

Project description:Tomato fruit ripening is associated with a dramatic increase in susceptibility to the fungal pathogen Botrytis cinerea, the causal agent of gray mold. Mature green fruit, prior to ripening, are largely resistant to B. cinerea, whereas red fruit, at the end of ripening, are susceptible to B. cinerea infection. We used microarrays to detail the gene expression changes that are induced by B. cinerea when tomato fruit at unripe and ripe stages are infected. Experiment Overall Design: Tomato fruit at mature green and red ripe stages were wound inoculated with a water suspension of B. cinerea conidia. Twenty four hours post inoculation fruit pericarp and epicarp tissue around and including the inoculation sites was collected and the total RNA extracted. Total RNA was also collected from healthy and mock inoculated fruit.

2009-06-01 | E-GEOD-14637 | biostudies-arrayexpress

Next generation sequencing analysis of Arabidopsis thaliana transcriptomes in response to Botrytis cinerea infection

Project description:Next generation sequencing was performed to identify genes changed in Arabidopsis thaliana upon Botrytis cinerea infection. The goal of the work is to find interesting genes involved in plant defense. The object is to reveal the molecular mechanism of plant defense.

2022-07-20 | GSE186842 | GEO

WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation

Project description:The Arabidopsis thaliana mutant wrky33 is highly susceptible to the necrotrophic fungus Botrytis cinerea. Comparing the expression profiles of B. cinerea-infected wrky33 and WT plants we identified 2765 differentially expressed genes dependent on WRKY33, of which 1675 were up-regulated in the mutant (termed WRKY33-repressed genes) and 1090 were down-regulated in the mutant. Combined with ChIP-seq data 318 genes were identified as direct functional targets of WRKY33 at 14 h post inoculation with spores of Botrytis cinerea 2100. Comparison of altered gene expression in Arabidopsis WT and wrky33 mutant plants 14 hours post inoculation with Botrytis cinerea 2100.

2015-06-15 | E-GEOD-66290 | biostudies-arrayexpress

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