KDM6A Regulates Immunogenicity of Multiple Myeloma (ChIP-seq)

ABSTRACT: We created isogenic mutiple myeloma and mouse embryonic fibroblast cell lines disrupted for KDM6A using CRISPR editing and Cre-mediated deletion. To detect KDM6A we inserted a dual HA tag sequence into the C-terminal of KDM6A by CRISPR-mediated editing. We also created one isogenic cell line harboring a jmjD-dead KDM6A where 2 amino acids essential for KDM6A enzymatic activity, H1146 and E1148 , were replaced with alanine residues

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE244510 | GEO | 2024/10/01

REPOSITORIES: GEO