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Transcriptome analysis of the mouse fetal and adult rete ovarii and surrounding tissues

ABSTRACT: The rete ovarii (RO) is an epithelial structure that arises during fetal development in close proximity to the ovary, and persists throughout adulthood in mammals. However, the functional significance of the RO remains elusive and it has been absent from recent discussions of female reproductive anatomy. The RO comprises three distinct regions: the intraovarian rete (IOR) within the ovary, the extraovarian rete (EOR) in the periovarian tissue, and the connecting rete (CR) linking the EOR and IOR. We hypothesize that the RO plays a pivotal role in maintaining ovarian homeostasis and responding to physiological changes. To uncover the nature and function of RO cells, we conducted transcriptome analysis, encompassing bulk, single-cell, and nucleus-level sequencing of both fetal and adult RO tissues using the Pax8-rtTA; Tre-H2B-GFP mouse line, where all RO regions express nuclear GFP. This study presents three datasets, which highlight RO-specific gene expression signatures and reveal differences in gene expression across the three RO regions during development and in adulthood. The integration and rigorous validation of these datasets will advance our understanding of the RO's roles in ovarian development, female maturation, and adult female fertility.

ORGANISM(S): Mus musculus

PROVIDER: GSE244849 | GEO | 2023/11/07

REPOSITORIES: GEO

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