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Whole-genome analysis of transcription-coupled repair reveals novel transcription events in Caenorhabditis elegans

ABSTRACT: We have adapted the eXcision Repair-sequencing (XR-seq) method to generate single-nucleotide resolution dynamic repair maps of UV-induced cyclobutane pyrimidine dimers (CPD) photoproducts in the Caenorhabditis elegans (C. elegans) genome. We focus on the C. elegans ortholog of the human XPC-deficient strain (xpc-1) and its exclusive use of transcription-coupled repair. We provide evidence demonstrating the utility of xpc-1 XR-seq as a remarkable tool for detecting nascent transcription and identifying new transcripts. The integration of epigenetic markers, chromatin states, and non-coding RNA annotations supports the robust detection of intergenic nascent transcription by XR-seq. Overall, our results provide a comprehensive view of the transcription-coupled repair landscape in C. elegans, highlighting their potential contributions to our understanding of DNA repair mechanisms and non-coding RNA biology.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE245181 | GEO | 2023/11/21

REPOSITORIES: GEO

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Genome-wide analysis of human global and transcription-coupled excision repair of UV damage at single-nucleotide resolution

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