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Expression analysis of transgenic embryos carrying the mitf-BRAFV600E allele in the presence or absence of p53 function

ABSTRACT: Investigation of expression differences between melanomas harvested from MiniCoopR-GFP versus MiniCoopR-SETDB1 transgenic zebrafish The embryos described in this study are further analyzed in a manuscript submitted for publication by White, et al.

ORGANISM(S): Danio rerio

PROVIDER: GSE24529 | GEO | 2011/03/30

SECONDARY ACCESSION(S): PRJNA132667

REPOSITORIES: GEO

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Expression analysis of transgenic embryos carrying the mitf-BRAFV600E allele in the presence or absence of p53 function

Project description:Investigation of expression differences between melanomas harvested from MiniCoopR-GFP versus MiniCoopR-SETDB1 transgenic zebrafish The embryos described in this study are further analyzed in a manuscript submitted for publication by White, et al. A 12 chip study comparing gene expression levels in 80hpf animals either WT (Tu), mitf-BRAFV600E, p53-/-, or mitf-BRAFV600E;p53-/- (double)

2011-03-30 | E-GEOD-24529 | biostudies-arrayexpress

Expression analysis of zebrafish melanoma and skin from the mitf-BRAFV600E;p53-/- line

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Expression analysis of zebrafish melanoma and skin from the mitf-BRAFV600E;p53-/- line

Project description:Investigation of expression differences between skin and melanomas from a transgenic BRAFV600E zebrafish model of melanoma The embryos described in this study are further analyzed in a manuscript submitted for publication by White, et al. A 15 chip study using RNA extracted from either WT zebrafish skin, mitf-BRAFV600E;p53-/- skin or mitf-BRAFV600E;p53-/- melanoma

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Expression analysis of melanoma harvested from GFP versus SETDB1 transgenic zebrafish (Danio rerio)

Project description:Investigation of expression differences between melanomas harvested from MiniCoopR-GFP versus MiniCoopR-SETDB1 transgenic zebrafish. An eight-chip study using total RNA prepared from four distinct melanomas from zebrafish injected with MiniCoopR-GFP (control) transposon and four distinct melanomas from zebrafish injected with MiniCoopR-SETDB1 transposon. Injected animals carried a p53 loss-of-function mutation, a mutation in nacre, and an mitf:BRAF-V600E transgene. Each chip measures the expression level of 32,292 genes.

2011-01-07 | E-GEOD-21505 | biostudies-arrayexpress

Expression analysis of melanoma harvested from GFP versus SETDB1 transgenic zebrafish (Danio rerio)

Project description:Investigation of expression differences between melanomas harvested from MiniCoopR-GFP versus MiniCoopR-SETDB1 transgenic zebrafish.

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The histone methyltransferase SETDB1 contributes to melanoma tumorigenesis and serves as a new potential therapeutic target in BRAF/MEK inhibitor-resistant melanoma [ChIP-seq]

Project description:Histone modifications play a crucial role in the progression of various cancers. The histone methyltransferase SETDB1 catalyzes the addition of methyl groups to histone H3 at lysine 9. Here, we describe SETDB1 contribution to melanoma tumorigenesis. SETDB1 is highly amplified in melanoma cells and in patients’ tumors. Increased SETDB1 expression, which correlates with SETDB1 amplification, is associated with a more aggressive phenotype in in vitro and in vivo studies. SETDB1 implements its effects through the regulation of Thrombospondin 1. SETDB1’s SET-domain is essential to maintain its tumorigenic effects. SETDB1 inhibition reduces cell growth in melanomas resistant to targeted treatments. In essence, we support SETDB1 as a major driver of melanoma development, highlighting a role as potential future target for the treatment of this disease.

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Timecourse gene expression microarray analysis of bi-allelic targeted Setdb1 mutant mouse ES cells, comparing cells with one inducible conditional (floxed) null allele and a second constitutive null allele, to control mouse ES cell lines.

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Analysis of cisplatin exposure on the kidney cortex proteome in the presence and absence of OCT1/2

Project description:WT and mOCT1/2 knockout mice (FVB background) were treated with NaCl or Cisplatin (4 mg/kg bodyweight i.p.) for 4 weeks. The kidneys were perfused and analysed via nLC-MS/MS. Please see the respective manuscript for details.

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