Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq analysis of early T cell progenitors from various gene deficient mice

ABSTRACT: Analysis of the gene expressions of early T progenitors in the lymphocyte development.

ORGANISM(S): Mus musculus

PROVIDER: GSE245400 | GEO | 2025/03/12

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

ATAC-seq analysis of early T cell progenitors from various gene deficient mice

Project description:Analysis of epigenetics of early T progenitors in the lymphocyte development.

2025-03-12 | GSE245399 | GEO

Early T cell progenitors from various gene deficient mice

Project description:Early T cell progenitors from various gene deficient mice

| PRJNA1028276 | ENA

RNA-seq analysis of early T cell progenitors from various gene deficient mice

Project description:RNA-seq analysis of early T cell progenitors from various gene deficient mice

| PRJNA1028278 | ENA

ATAC-seq analysis of early T cell progenitors from various gene deficient mice

Project description:ATAC-seq analysis of early T cell progenitors from various gene deficient mice

| PRJNA1028279 | ENA

Early T cell progenitors from various gene deficient mice

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-03-12 | GSE245401 | GEO

Transcriptional deregulation in EZH2-deficient lymphocyte progenitors

Project description:This goal of this study was to identify genes that are deregulated in the absence of EZH2 in early lymphocyte progenitors. We examined gene expression by RNA-sequencing in sorted CLPs (Lin-CD117int CD127+ CD135+), pro-B cells (B220+CD19+CD43+), DN3 cells (Lin- CD25+ CD117-), splenic NK cells (Lin-NK1.1+DX5+) and bone marrow ILC2 cells (Lin- Sca1+CD127+) from Ezh2fl/fl Il7racre/+ and Il7racre/+ control mice. Reads were aligned to the mm10 reference genome by Tophat2.1.0. Reads were assigned to genes using the htseq-count tool from HTSeq v 0.6.1 and gene annotations from Ensembl release 78. Differential expression was calculated across 2-3 independent replicates by EdgeR. We found that CLPs, ILC2s, and splenic NK cells maintained their normal transcriptional programs despite loss of EZH2. In contrast, loss of EZH2 caused over 1000 genes to be deregulated in pro-B and DN3 cells indicating that EZH2 is required for transcriptomic stability in adaptive, but not innate lymphocyte progenitors.

2017-04-07 | GSE97462 | GEO

RNA-seq of CDKN2A and EZH2-deficient pro-B and DN3 lymphocyte progenitors

Project description:This goal of this study was to identify genes that are deregulated in the absence of EZH2 in early lymphocyte progenitors. Due to a requirement for EZH2 to repress Cdkn2a in early B and T cell development we generated Cdkn2a-/-Ezh2fl/fl Il7racre/+ mice. We examined gene expression by RNA-sequencing in sorted pro-B cells (B220+CD19+CD43+), DN3 cells (Lin- CD25+ CD117-), from Cdkn2a-/- Ezh2fl/fl Il7racre/+ and Cdkn2a-/- Il7racre/+ control mice. Reads were aligned to the mm10 reference genome by Tophat2.1.0. Reads were assigned to genes using the htseq-count tool from HTSeq v 0.6.1 and gene annotations from Ensembl release 78. Differential expression was calculated across 3 independent replicates by EdgeR. We found that pro-B and DN3 cells remain specified to their respective lineages despite loss of EZH2. In contrast, loss of EZH2 led to expression of alternate lineage determinants in pro-B but not DN3 cells indicating that EZH2 is required for lineage commitment in B, but not T lymphocyte progenitors.

2020-01-29 | GSE133993 | GEO

Characterisation of early thymic progenitors and thymus seeding progenitors

Project description:Gene expression analysis of early thymic progenitors and thymus seeding progenitors

2012-02-19 | GSE29382 | GEO

Characterisation of early thymic progenitors and thymus seeding progenitors

Project description:Gene expression analysis of early thymic progenitors and thymus seeding progenitors Eight distinct populations were analysed, each with between 2 and 6 biological replicates.

2012-02-19 | E-GEOD-29382 | biostudies-arrayexpress

Transcriptome analysis of lymphoid progenitors in Zbtb1-deficient mice

Project description:Purpose: Deficiency of Zbtb1 leads to the generation of myeloid cells from lymphoid progenitors when cultured in conditions that do not support myeloid development. We therefore analyzed how was the transcriptional signature altered by Zbtb1 deficiency in lymphoid progenitors ex vivo and after induction of a T-cell developmental program by co-culture with OP9-DL1 stroma cells.

2017-07-28 | GSE84961 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data