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Screening and characterizing the effects of mmu_circ_0000296 on spinal cord injury

ABSTRACT: Spinal cord injury (SCI) is a common pathologic disorder that often presents with sensory and motor deficits, severely affecting patients' daily lives. Circular RNAs (circRNAs), a class of non-coding RNAs, have been reported to be involved in regulating the development of SCI. We used RNA sequencing technology and bioinformatics analysis to find the expression and regulation patterns of circRNAs and mRNAs in a mouse model of SCI, from which we screened the potential RNA-mmu_circ_0000296 that affects the progression of SCI in the acute phase. Based on STRING, ENCORI, miRTarBase database and Cytoscape software to construct the relational network of endogenous competing RNAs (ceRNAs) and protein-protein interaction (PPI) network. Finally, in order to validate the above bioinformatics results, the differential expression of circ_0000296 was verified in mouse SCI model and LPS-induced cell model, respectively. Not only that, in in vitro experiments, we found that circ_0000296 overexpression could inhibit microglia polarization toward M1 type, reduce inflammation level, and then inhibit neuronal apoptosis. These findings will help to elucidate and refine the effects of circRNAs on the pathophysiological progression of spinal cord injury and suggest that mmu_circ_0000296 may be one of the targets for spinal cord injury therapy.

ORGANISM(S): Mus musculus

PROVIDER: GSE245437 | GEO | 2023/10/31

REPOSITORIES: GEO

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