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Bulk RNA-sequence of four monocyte-macrophage subpopulations isolated from the IgE-dependent skin allergic inflammation (IgE-CAI) skin lesion

ABSTRACT: Basophils play critical roles in the development of mouse delayed-onset skin allergic inflammation (IgE-CAI model). Importantly, they also contribute to the resolution of allergic inflammation by promoting the generation of pro-resolving macrophages. However, it remains unclear how pro-resolving macrophages suppress excess inflammation. To address this, we conducted single-cell RNA-seq (scRNA-seq) analysis of the IgE-CAI skin lesion at days 3 and 5 post-challenge of allergens. scRNA-seq analysis identified four distinct monocyte-macrophage subpopulations, namely classical monoytes, early CMDMs, late CMDMs and resident-like macrophages. Based on the gene expression profiles, classical monoytes, early CMDMs, late CMDMs and resident-like macrophages corresponded to Ly6ChiPD-L2lo, Ly6ChiPD-L2hi, Ly6CloPD-L2hi, and Ly6CloPD-L2lo, respectively.

ORGANISM(S): Mus musculus

PROVIDER: GSE245865 | GEO | 2024/01/22

REPOSITORIES: GEO

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Single-cell RNA-sequence of the skin lesion of IgE-dependent skin allergic inflammation (IgE-CAI) model

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