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The GLI-code controls HNF1A levels during in-vitro foregut differentiation

ABSTRACT: Differentiation of human induced pluripotent stem cells towards pancreatic islet endocrine cells is a complex process, involving the stepwise modulation of key developmental pathways, such as the Hedgehog signaling inhibition during early differentiation stages. In tandem with this active inhibition, key transcription factors for the islet endocrine cell fate, such as HNF1A, show specific changes in their expression patterns. Here we designed a forthright pilot study aimed at investigating the potential interconnection between HH-signaling inhibition and the increase in the HNF1A expression during early regeneration, by inducing changes in the GLI code. This unveiled a link between the two, where GLI3-R mediated Hedgehog target genes inhibition is apparently required for HNF1A efficient expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE246049 | GEO | 2023/10/27

REPOSITORIES: GEO

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Identification of GLI-associated proteins by mass spectrometry

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