Genomics

Dataset Information

0

Single-cell transcriptomic analysis of lung mesenchymal cells with mesenchyme-specific deletion of transcriptional regulator Sin3a


ABSTRACT: Congenital diaphragmatic hernia (CDH) is a common and severe congential malformation characterized by defects in diaphragm, lung, and pulmonary vascular developent. Despite the frequency and severity of CDH, the underlying developmental mechanisms are not understood. We identified SIN3A loss of function sequence variants in two patients with CDH. To understand the genetic and developmental mechanisms of CDH, we generated Sin3a conditional knockout mice that lack Sin3a expression in the lung mesenchyme. SIN3A has been shown to play a critcal role during development, directing cell lineage specification and cell cycling. We found that loss of SIN3A resulted in impaired mesenchymal cell differentiation from bulk lung transcriptomic analysis in Sin3a CKO mice. To investigate the impact of loss of SIN3A in mesenchymal cell differentation, we performed fluorescent activated cell sorting (FACS) to isolate cells that underwent recombination of Sin3a. We then performed single-cell transcriptomic analysis on sorted mesenchymal cells from embryonic day 16 (E16) Sin3a CKO and control lungs. In this dataset are expression data from FACS-isolated recombined lung mesenchymal cells of Sin3a CKO and control mice. Sin3a CKO mice have conditional deletion of Sin3a in the lung mesenchyme directed by Tbx4rtta; tetocre (Tbx4rtta; tetocre; Sin3a flox/flox CKO). Control mice are heterozygous for Sin3a in the lung mesenchyme (Tbx4rtta; tetocre; Sin3a flox/WT). These data were used to identify transcriptional changes due to loss of Sin3a in the lung mesenchyme.

ORGANISM(S): Mus musculus

PROVIDER: GSE246194 | GEO | 2023/12/31

REPOSITORIES: GEO

Similar Datasets

2023-12-31 | GSE246184 | GEO
2023-12-31 | GSE246305 | GEO
2017-11-02 | GSE106399 | GEO
2017-06-22 | GSE94306 | GEO
2020-08-31 | GSE139818 | GEO
2022-07-19 | GSE206449 | GEO
2014-09-02 | E-GEOD-51643 | biostudies-arrayexpress
2022-07-19 | E-MTAB-11888 | biostudies-arrayexpress
2020-06-28 | E-MTAB-9235 | biostudies-arrayexpress
2013-12-09 | E-GEOD-51283 | biostudies-arrayexpress