Dataset Information

Analysis of global gene expression using RNA-sequencing reveals new roles of Yanghe Pingchuan decoction in asthma

ABSTRACT: Context: Yanghe Pingchuan decoction (YPD) has been used for asthma treatment for many years in China. Objective: We sought to understand the role of YPD, and find more potential targets for YPD-based treatment of asthma. Materials and methods: An ovalbumin-induced asthma model in rats was created. Staining (hematoxylin and eosin, Masson) was used to evaluate the treatment effect of YPD. RNA-sequencing was carried out to analyze global gene expression, and differentially expressed genes (DEGs) were identified. Analysis of the functional enrichment of genes was done using the Gene Ontology database. Analysis of signaling-pathway enrichment of genes was done using the Kyoto Encyclopedia of Genes and Genomes database. Real-time reverse transcription-quantitative polymerase chain reaction was undertaken to measure expression of DEGs. Results: Pathology showed that YPD had an improvement effect on rats with asthma. RNA-sequencing showed that YPD led to upregulated and downregulated expression of many genes. The YPD-based control of asthma pathogenesis may be related to calcium ion (Ca2+) binding, inorganic cation transmembrane transporter activity, microtubule motor activity, and control of canonical signaling (e.g., peroxisome proliferator-activated receptor, calcium, cyclic adenosine monophosphate). Enrichment analyses suggested that asthma pathogenesis may be related to Ca2+ binding and contraction of vascular smooth muscle. A validation experiment showed that YPD could reduce the Ca2+ concentration by inhibiting the Ang-Ⅱ/PLA/CaM signaling axis. Discussion and conclusion: Control of asthma pathogenesis by YPD may be related to inhibition of the Ang-Ⅱ/PLA/CaM signaling axis, reduction of the Ca2+ concentration, and relaxation of airway smooth muscle.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE247032 | GEO | 2023/11/07

REPOSITORIES: GEO

ACCESS DATA

Similar Datasets

Project description:Systemic lupus erythematous (SLE) is a prototype of autoimmune disease. Lupus nephritis (LN) is one of the most serious complications of SLE. The development of autoreactive B cells and the production of autoantibodies have been critical for the initiation and progression of LN. Store-operated Ca2+ entry (SOCE) is the main Ca2+ influx pathway in lymphocytes and is essential for immune response . SOCE is mediated by Ca2+ release-activated Ca2+ (CRAC) channels which are comprised of stromal interaction molecule (STIM) and calcium release-activated calcium modulators (ORAI) . Mutations in genes encoding the CRAC channel abolish SOCE in cells of the immune system and cause severe combined immunodeficiency . Calcium signaling via ORAI1 has been involved in the pathogenesis of autoimmune diseases by driving Th17 differentiation . STIM1 deficiency significantly reduced Th1/Th17 responses and resulted in complete protection from experimental autoimmune encephalomyelitis . Compared to T cells, the roles of CRAC channel in B cells is far less clear. Ca²⁺/calmodulin (CaM)-dependent protein kinase2 (CaMK2) is a serine/threonine-specific protein kinase that is regulated by the Ca²⁺/CaM complex. CaMK2 has been involved in many signaling pathways and is necessary for Ca²⁺ homeostasis, T cell development and activation. CaMK4, another member of the CaMK family, has been shown to compromises podocyte function and promote renal diseases in LN. In the current study, we found that CRAC channel mediated calcium signaling is enhanced in B cells from patients with LN. CRAC channel inhibition by YM-58483 and knocked down of CRAC channel by ORAI1 or STIM2 siRNA led to suppression of CaMK2 signaling and decreased B cell differentiation. Lupus mice treated with CRAC channel inhibitor showed reduced anti-double stranded DNA antibodies (anti-dsDNA), decreased immune deposition in the glomeruli and improved renal function. CRAC channel mediates the development and progression of LN by promoting the differentiation of B cells into plasma cells.

Dataset Information

Analysis of global gene expression using RNA-sequencing reveals new roles of Yanghe Pingchuan decoction in asthma

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure