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Campylobacter jejuni comparative genome: control strain C. jejuni subsp. jejuni ATCC 700819 (NCTC 11168) vs. 12 other strains

ABSTRACT: Gene content comparison of control C. jejuni subsp. jejuni strain 11168 which colonizes and causes disease in C57BL/6 IL-10-/- mice versus C. jejuni strains D6844, D6845, D6846, D6847, D6848, D6849, D0121, D0835, D2586, D2600,33560 and NW in the C57BL/6 IL-10-/- mice. Keywords: DNA/DNA comparison

ORGANISM(S): Campylobacter jejuni

PROVIDER: GSE24764 | GEO | 2010/10/21

SECONDARY ACCESSION(S): PRJNA132205

REPOSITORIES: GEO

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Campylobacter jejuni comparative genome: control strain C. jejuni subsp. jejuni ATCC 700819 (NCTC 11168) vs. 12 other strains

Project description:Gene content comparison of control C. jejuni subsp. jejuni strain 11168 which colonizes and causes disease in C57BL/6 IL-10-/- mice versus C. jejuni strains D6844, D6845, D6846, D6847, D6848, D6849, D0121, D0835, D2586, D2600,33560 and NW in the C57BL/6 IL-10-/- mice. Keywords: DNA/DNA comparison Two genome comparison of disease strain versus non disease strain of C.j., 4 Biological replicates - 2 of which were dye swaps

2010-10-21 | E-GEOD-24764 | biostudies-arrayexpress

Campylobacter jejuni comparative genome: Control strain 11168 vs. strain NW

2008-12-03 | GSE13794 | GEO

Differential RNA-sequencing of Campylobacter jejuni NCTC 11168

Project description:We report the use of differential RNA-sequencing for the determination of the primary transcriptome of wildtype Campylobacter jejuni NCTC 11168. This allows for the genome-wide determination of transcription start sites.

2013-09-13 | GSE49312 | GEO

Campylobacter jejuni comparative genome: Control strain 11168 vs. strain NW

Project description:Gene content comparison of control C.j. strain 11168 which colonizes and causes disease in a murine model versus strain NW which colonizes but does not elicit disease symptomology in the mouse model. Keywords: DNA/DNA comparison Two genome comparison of disease strain versus non disease strain of C.j., Biological replicates - 2, independently grown and harvested, Technical replicates 2, also independent, 3 ORF replicates per array.

2010-05-15 | E-GEOD-13794 | biostudies-arrayexpress

RNA-sequencing of Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant

2014-07-01 | GSE49687 | GEO

Screening for novel immunogenic proteins of Campylobacter jejuni NCTC 11168

Project description:The screening of a cDNA derived expression library of Campylobacter jejuni NCTC 11168 expressed in E. coli using a fusion construct and specific HaloTag interaction to a modified surface is shown. 1536 different clones were screened including positive (hisJ, cjaA, peb1a) and negative (argC, pyrC, gapA) reference proteins. The goal of the screening was to identify potential novel immunogenic proteins from C. jejuni by selecting clones showing a high signal intensity in comparison to the known antigens used as positive markers. Afterwards, the most promising clones were sequenced to identify the gene and corresponding protein, and these proteins were then investigated further. Consequently, 22 novel immunogenic proteins could be identified.

2013-04-06 | GSE45268 | GEO

Campylobacter jejuni subsp. jejuni NCTC 11168-PO

Project description:Campylobacter jejuni subsp. jejuni NCTC 11168-PO Genome sequencing

| PRJNA159829 | ENA

RNA-sequencing of Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant

Project description:We report the use of RNA-seq analysis for the determination of RPKM transcript levels in wildtype and fur perR mutant of Campylobacter jejuni NCTC 11168. This allows for comparison of gene expression levels. Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant were grown to late log phase, RNA was purified and used for RNA-sequencing by Illumina HiSeq sequencing

2014-07-01 | E-GEOD-49687 | biostudies-arrayexpress

Transcriptional profiles of Campylobacter jejuni NCTC 11168 to 4mg/L erythromycin treatment

Project description:Erythromycin is the drug of choice to treat campylobacteriosis, but resistance to this antibiotic is rising. The adaptive mechanisms employed by Campylobacter jejuni to erythromycin treatment remain unknown. The aim of this study is to determine the molecular basis underlying CampylobacterM-bM-^@M-^Ys immediate response to Ery treatment. The design utilized an available two color microarray slide for the entire transcriptome of Campylobacter jejuni wild type strain NCTC 11168. One hybridizations were performed: sham-treated NCTC 11168 v.s. lethal dose erythromycin treated NCTC 11168. Samples were independently grown and harvested. There were three biological replicates of each sample.

2013-10-10 | E-GEOD-43023 | biostudies-arrayexpress

Transcriptional profiles of Campylobacter jejuni NCTC 11168 to 0.125mg/L erythromycin treatment

Project description:Erythromycin is the drug of choice to treat campylobacteriosis, but resistance to this antibiotic is rising. The adaptive mechanisms employed by Campylobacter jejuni to erythromycin treatment remain unknown. The aim of this study is to determine the molecular basis underlying CampylobacterM-bM-^@M-^Ys immediate response to Ery treatment. The design utilized an available two color microarray slide for the entire transcriptome of Campylobacter jejuni wild type strain NCTC 11168. One hybridizations were performed: sham-treated NCTC 11168 v.s. sub-lethal dose erythromycin treated NCTC 11168. Samples were independently grown and harvested. There were three biological replicates of each sample.

2013-10-10 | E-GEOD-43024 | biostudies-arrayexpress

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