Spatial total RNA sequencing of formalin-fixed paraffin embedded tissue by Spatial Random-seq

ABSTRACT: Current spatial transcriptomic methods have been widely used to resolve gene expression; however, these methods are limited to fresh or fresh-frozen samples due to unsuccess of oligo(dT) primers in degraded RNA samples. Here we develop a spatial random-sequencing (spRandom-seq) technology for Formalin-fixed paraffin-embedded (FFPE) tissues by capturing full-length total RNAs with random primers. This approach provides a powerful spatial platform for clinical FFPE specimens and promises enormous applications in biomedicine.

ORGANISM(S): Mus musculus

PROVIDER: GSE247881 | GEO | 2023/12/31

REPOSITORIES: GEO