Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell RNA-seq of single cell suspension from the HCC region of WT and FABP5 Hepatocyte KO mice fed CD-HFD for 15 month

ABSTRACT: After feeding CD-HFD diet for 15 month, tumors from WT and FABP5 hepatocyte KO mice were collected and digested with collagenase to generate single cell suspension before proceeding with 10X Single Cell RNA-Sequencing.

ORGANISM(S): Mus musculus

PROVIDER: GSE248010 | GEO | 2024/02/23

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Single cell RNA-seq of single cell suspension from the HCC region of mice fed CD-HFD for 15 mice and treated with FABP5 inhibitor or control

Project description:We perform scRNA-seq in the livers of C57BL/6 mice fed a CD-HFD for 15 months. Starting from the 13M, mice were treated with FABP5 inhibitor to ameliorate HCC.

2024-02-17 | GSE231818 | GEO

High-throughput sequencing analysis of mouse FABP5 KO BMDM cell transcriptomes with overexpression of FABP5 WT or FABP5 C127S after LPS treatment

Project description:Purpose: Mouse BMDM is the universal cell type of studying innate immunity. This study was designed to analyze LPS induced innate immune response and the gene expression in FABP5 KO BMDMs with overexpression of FABP5 WT or FABP5 C127S. Methods: FABP5 KO BMDMs were nucleofected with 5 μg pXJ40-3xFlag-FABP5 WT or C127S plasmid using the Amaxa Mouse Macrophage Nucleofector Kit (Lonza, VPA-1009) following the manufacturer’s instructions. Replace medium 6 hours post Nucleofection and add 500 ng/mL LPS to the fresh medium. 24 hours after treatment, harvest cells by using GenElute Single Cell RNA Purification Kit (Sigma, RNB300) and perform RNA-seq. Then mRNA profiles of these samples were generated by high-throughput sequencing analysis, using Illumina NovaSeq6000. And the differential mRNA profiles were analyzed. Results: mRNA profiles were analyzed, and differential expression profiles were compared. Conclusions: Our study demonstrated the transcriptional profiles of FABP5 KO BMDMs with overexpression of FABP5 WT or FABP5 C127S upon LPS treatment, with biologic replicates, generated by RNA-seq technology.

2021-11-01 | GSE182238 | GEO

Gastric cancer cells exploit FABP5 via the PD-L1/TNF/IL-17 signaling axis

Project description:Background: Gastric cancer (GC) is the second most lethal cancer globally and is associated with poor prognosis. Fatty acid-binding proteins (FABPs) can regulate the biological properties of carcinoma cells. FABP5 is overexpressed in many types of cancers; however, the role and mechanism of FABP5 in GC are still unclear. Aim: In this study, we aimed to evaluate the clinical and biological function of FABP5 in GC. Methods: We assessed FABP5 expression using immunohistochemical analysis of 79 GC patients and evaluated its biological functions following in vitro and in vivo ectopic expression. FABP5 targets relevant to GC progression were determined using RNA sequencing (RNA-seq) analyses. Results: Elevated FABP5 expression was closely related to poor outcomes, and ectopic expression of FABP5 promoted GC cell proliferation, invasion, migration, and carcinogenicity, suggesting its potential tumor-promoting role in GC. Additionally, RNA-seq analysis indicated that FABP5 activates immune-related pathways, including cytokine–cytokine receptor-interaction pathways, interleukin-17 signaling, and tumor necrosis factor signaling, suggesting an important rationale for the possible development of therapies combining FABP5-targeted drugs with immunotherapeutics. Conclusion: These findings highlight the biological mechanisms and clinical implications of FABP5 in GC and suggest its potential as an adverse prognostic factor and/or therapeutic target.

2023-12-31 | GSE218422 | GEO

Down-regulation of FABP5 suppresses the proliferation and induces apoptosis in gastric cancer cells through Hippo signaling pathway

Project description:We used RNA-seq to study the function of FABP5 in gastric cancer cells (AGS and MGC803). Down-regulation of FABP5 suppressed cell proliferation, cell migration and invasion, and induced cell apoptosis. Bioinformatics analysis revealed that Hippo signaling pathway was related to FABP5 in GC cells.Our data suggested that FABP5 might act as a potential target associated with Hippo signaling pathway for GC treatment.

2022-12-31 | GSE171838 | GEO

High-throughput sequencing analysis of mouse FABP5 KO BMDM cell transcriptomes with overexpression of FABP5 WT or FABP5 C127S after LPS treatment

Project description:High-throughput sequencing analysis of mouse FABP5 KO BMDM cell transcriptomes with overexpression of FABP5 WT or FABP5 C127S after LPS treatment

| PRJNA755440 | ENA

Effect of exogenous FABP4 (A-FABP/aP2) and FABP5 (E-FABP/mal1) on gene expression in adipocyte-derived stem cells (ADSC) and 233A cells

Project description:Gene expression profile of treatment with FABP4 or FABP5 in ADSC and 233A cells was examined. ADSC or 233A cells were treated with and without 1 ÂµM recombinant FABP4 or FABP5.

2016-06-23 | E-GEOD-83587 | biostudies-arrayexpress

Mitochondrial integrity regulated by FABP5 is a cell-intrinsic checkpoint for Treg suppressive function

Project description:Regulatory T cells (Tregs) negatively regulate immune-mediated inflammation that is essential for preventing autoimmunity, but which can be detrimental in cancer. Central to Treg activation are changes in lipid metabolism to support their survival and function. Fatty acid binding proteins (FABPs) are a family of lipid chaperones required to facilitate the uptake and trafficking of intracellular lipids. One family member, FABP5, is expressed in certain T cell subsets, but its function remains elusive. We show here that in Tregs, FABP5 inhibition causes mitochondrial defects, underscored by decreased OXPHOS, lipid elongation and desaturation, and loss of cristae structure, which augment their suppressive function. Mitochondrial dysfunction after FABP5 inhibition results in mtDNA release and consequent cGAS/STING-dependent type I IFN signaling, which induces increased production of the regulatory cytokine IL-10, promoting Treg cell suppressive activity. Together these data reveal that FABP5 acts as a gatekeeper of mitochondrial health to control Treg cell function.

2019-12-26 | GSE126245 | GEO

p38MAPKa stromal reprogramming sensitizes metastatic breast cancer to immunotherapy

Project description:Femurs bearing breast metastatases (5-pooled animals per sample) were crushed and digested with 2mg/ml Collagenase A.

2023-03-08 | GSE210286 | GEO

Effect of FABP5 silencing or inhibition on gene expression of Huh7 human hepatoma cells after 48 hours

Project description:To investigate the function of FABP5 in the regulation of metabolic reprogramming in Huh7 cells in which target gene has been silenced by siRNA or inhibited by competitive inhibitor SBFI-103.

2024-02-17 | GSE228430 | GEO

Gene array analysis of langerhans cells and macrophages from human skin and pDCs from human spleen

Project description:Langerhans cells and macrophages were isolated from adult skin that was collagenase digeseted overnight by FACS. Adult spleen was collagenase digested for 1hr and the pDCs were isolated by FACS. Gene expression analysis using total RNA from specific cell subsets was performed.

2020-01-21 | GSE122673 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data