3' RACE-seq of endogenous LINE-1 mRNA's 3' non-templated ends in PA-1 control kd, XRN1 kd, DCP2 kd, and DIS3L2 kd (kd by RNAi for 48h = set 1, or 126h = set 2), and 293T wild-type and XRN1 KO.

ABSTRACT: Human LINE-1 retrotransposons can mobilize in the human genome by a copy-and-paste machanism involving RNA intermediates. LINE-1 mRNA 3' ends play a major role in initiation of reverse transcription (a step retrotransposition). To assess the impact of RNA 3’ end dynamics of LINE-1 mRNA on its retrotransposition potential in human cells we analyzed 3' non-templated ends on LINE-1 mRNA by 3' RACE-seq. RNA was retrieved from multiple clonal 293T and PA-1 cells wild-type or knock-out in either XRN1, DCP2, or DIS3L2. Publication: D Janecki, R Sen, N Szóstak, A Kajdasz, M Kordys, K Plawgo, D Pandakov, A Philips, Z Warkocki (2024): LINE-1 mRNA 3’ end dynamics shape its biology and retrotransposition potential. Nucleic Acids Research. https://doi.org/10.1093/nar/gkad1251

ORGANISM(S): Homo sapiens

PROVIDER: GSE248108 | GEO | 2024/01/05

REPOSITORIES: GEO