Project description:Liver has abundant regenerative capacity, but hepatocytes cannot be cultured long-term in vitro. Activation of FXR signaling induced iHOs with both hepatocyte traits and proliferative potential. To investigate the transcriptomic analysis of iHOs, we performed bulk RNA-seq analysis of organoids in different passages and single cell RNA-seq analysis of iHOs. Compared to 2D-iPS-Heps and organoids in other culture conditions, iHOs have expression profiles more similar to primary human hepatocytes and human fetal liver cells. Single cell RNA-seq revealed iHOs have a fraction that co-expresses hepatocyte markers and proliferation markers.
Project description:To investigate the function of small heterodimer partner (SHP) in hepatocytes, we established Dox-inducible SHP overexpression iPSC cell lines and differentiated hepatocytes and cultured iPS-hepatocyte organoids (iHO). A comprehensive RNA-seq analysis was conducted. performed comparative gene expression analysis using data obtained from iHO vehicle and iHO dox.
Project description:Under iHO conditions, distinctive grape-like organoids emerge. To evaluate the hepatocyte characteristics of grape-like organoids and other organoid forms, grape-like organoids were isolated under a microscope, and RNA-seq was performed.
