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Plasmodesmal closure triggers stress responses

ABSTRACT: Plant cells function cell autonomously, but are connected to their cellular neighbors via plasmodesmata. Changes in cellular connectivity is required for cell signaling, with elicitor-induced plasmodesmal closure and consequent cellular isolation required for a full immune response. To understand what occurs when plasmodesmal closure is initiated, we generated two inducible transgenic lines (LexA::PD-Plug and LexA::icals3m). Inducing these genetic tools results in callose deposition at plasmodesmata, leading to plasmodesmal closure and cellular isolation, independent of a stimulus. We found that prolonged cellular isolation led to the upregulation of stress responsive genes and accumulation of salicylic acid, in addition to increased resistance to Pseudomonas syringae DC3000. We observed a more homogenous cellular isolation in LexA::icals3m lines in comparison to a heterogenous cellular isolation in LexA::PD-Plug, the latter which reflects more biological relevant observations in response stress elicitors. Interestingly, the LexA::icals3m displayed detrimental phenotypes with prolonged plasmodesmal closure in the form of hypersensitivity to Botrytis cinerea, perturbations of photoassimilate distribution with the accumulation of starch and sugars, and decrease in size of leaves and increased leaf senescence. These findings of what happens when a cell is isolated from its neighbors indicates we need to consider these factors in any context which plasmodesmata close, and also defines why cell-to-cell connectivity is regulated during key biological processes.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE248301 | GEO | 2026/03/05

REPOSITORIES: GEO

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