Surface-associated, motile populations of Pseudomonas aeruginosa gene regulation by Staphylococcus aureus exoproducts

ABSTRACT: Pseudomonas aeruginosa and Staphylococcus aureus are often co-isolated in persistent infections. The goal of this study was to determine how secreted products from S. aureus affect gene expression in surface-associated P. aerguinosa undergoing emergent motility. Therefore, media salts control or S. aureus supernatant was added to agar plates at 25% total volume. P. aeruginosa was inoculated on the agar and gene expression was measured from the leading edge after 17 h incubation using RNA-seq. P. aeruginosa moving on the agar containing S. aureus supernatant had an upregulation in genes involved in intermediate metabolite uptake and a downregulation in heme biosynthesis, response to heat, Type III Secretion System, and aerobic respiration pathways.

ORGANISM(S): Pseudomonas aeruginosa

PROVIDER: GSE249024 | GEO | 2024/02/09

REPOSITORIES: GEO