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Single-cell RNA-sequencing and paired TCR-sequencing of CD4⁺ and CD8⁺ T-cell differentiation in a mouse DNA vaccination model with or without CD4⁺ T-cell help

ABSTRACT: The data deposited in the GSE series support two independent studies that use a shared in vivo mouse vaccination model to trace activation and effector differentiation of endogenous polyclonal CD8⁺ and CD4⁺ T cells. Briefly, we employed a DNA vaccination model in C57BL/6 mice based on epidermal “tattoo” delivery of plasmid DNA encoding the HPV E7 (E7₄₈–₅₇) MHC-I epitope either alone or linked to three MHC-II helper epitopes (P30, PADRE, NEF). Vaccination on days 0, 3, and 6 induces antigen expression in keratinocytes and delivery to the draining lymph node (dLN) via migratory conventional dendritic cells and passive drainage. This model allows parallel tracking of antigen-specific CD8⁺ T cells (H-2Dᵇ/E7 tetramer) and CD4⁺ T cells (PADRE MHC-II tetramer), enabling controlled comparison of CD8⁺ T-cell responses in the presence or absence of CD4⁺ T-cell help, as well as mapping the differentiation trajectories of polyclonal CD4⁺ T cells. We provide single-cell RNA sequencing and paired TCR sequencing of CD3+CD44+CD62L- activated T cells isolated from pooled draining lymph nodes (dLN) and non-draining lymph nodes (ndLN) at days 5 and 10 post-vaccination.

ORGANISM(S): Mus musculus

PROVIDER: GSE249046 | GEO | 2026/03/13

REPOSITORIES: GEO

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