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Antagonistic stem cell fate under stress governs decisions between hair graying and melanoma [RNA-Seq]

ABSTRACT: The exposome, an individual’s lifelong environmental exposure, profoundly impacts health. Somatic tissues undergo functional decline with age, exhibiting characteristic ageing phenotypes including hair graying and cancer. However, the specific genotoxins, signals and cellular mechanisms underlying each phenotype remain largely unknown. Here, we report that melanocyte stem cells (McSCs) and their niche coordinately determine individual stem cell fate through antagonistic, stress-responsive pathways, depending on the type of genotoxic damage incurred. McSC fate-tracking in mice revealed that McSCs undergo cellular senescence-associated differentiation (seno-differentiation) in response to DNA double-strand breaks (DSBs), resulting in their selective depletion and hair graying, and effectively protecting against melanoma. Conversely, carcinogens can suppress McSC seno-differentiation, even in DSB-harboring cells, by activating arachidonic acid metabolism and the niche-derived KIT ligand, thereby promoting McSC self-renewal. Collectively, the fate of individual stem cell clones - expansion versus exhaustion - cumulatively and antagonistically governs ageing phenotypes through interaction with the niche.

ORGANISM(S): Mus musculus

PROVIDER: GSE249074 | GEO | 2025/07/07

REPOSITORIES: GEO

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Antagonistic stem cell fate under stress governs decisions between hair graying and melanoma [array]

Project description:The exposome, an individual’s lifelong environmental exposure, profoundly impacts health. Somatic tissues undergo functional decline with age, exhibiting characteristic ageing phenotypes including hair graying and cancer. However, the specific genotoxins, signals and cellular mechanisms underlying each phenotype remain largely unknown. Here, we report that melanocyte stem cells (McSCs) and their niche coordinately determine individual stem cell fate through antagonistic, stress-responsive pathways, depending on the type of genotoxic damage incurred. McSC fate-tracking in mice revealed that McSCs undergo cellular senescence-associated differentiation (seno-differentiation) in response to DNA double-strand breaks (DSBs), resulting in their selective depletion and hair graying, and effectively protecting against melanoma. Conversely, carcinogens can suppress McSC seno-differentiation, even in DSB-harboring cells, by activating arachidonic acid metabolism and the niche-derived KIT ligand, thereby promoting McSC self-renewal. Collectively, the fate of individual stem cell clones - expansion versus exhaustion - cumulatively and antagonistically governs ageing phenotypes through interaction with the niche.

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