Other

Dataset Information

0

A Whole-Cell Screening Platform for Discovering Cell Adhesion Molecules Enabling Programmable Bacterial Cell-Cell Adhesion


ABSTRACT: To identify cell adhesion molecules (CAMs) targeting bacterial membrane proteins within a synthetic bacteria-displayed nanobody library, we present a comprehensive whole-cell screening platform. This involves targeted amplicon sequencing to discover nanobodies targeting the natural adhesin, TraN. Furthermore, we employ deep mutational engineering to enhance the binding affinity of these nanobodies toward TraN.

ORGANISM(S): Escherichia coli

PROVIDER: GSE249103 | GEO | 2023/12/18

REPOSITORIES: GEO

Similar Datasets

2023-12-30 | E-MTAB-11396 | biostudies-arrayexpress
2021-09-21 | GSE158906 | GEO
2023-06-21 | GSE202972 | GEO
2023-11-16 | GSE223428 | GEO
2023-10-10 | GSE244714 | GEO
2024-01-26 | PXD046363 | Pride
2021-10-21 | GSE157485 | GEO
2021-10-21 | GSE157483 | GEO
2021-10-21 | GSE157478 | GEO
2024-01-26 | PXD032731 | Pride