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Dissection by single-cell RNA-sequencing of the cellular composition of mouse salivary gland epithelia (wild-type and irradiated).

ABSTRACT: The major salivary glands (SG) are exocrine glands responsible for the physiological production of saliva, a sero-mucinous fluid that plays a key role in the digestion and swallowing of food, the articulation of speech and the maintenance of oral hygene. Among the principal causes of SG dysfunction is radiation treatment of head-and-neck malignancies, which can cause severe damage to multiple cell-types within the SG epithelium, resulting in permanent loss of saliva production and substantial disability. The aim of this study is to analyze the cellular composition of the mouse SG epithelium using single-cell RNA-sequencing (scRNAseq) in order to: 1) gain a deeper understanding of the rich repertoire of different epithelial cell-types that coexist witin it; and 2) to elucidate whether different sub-types of epithelial cells display different radiosensitivity and are preferentially elimininated from SGs following radiotherapy. The dataset includes data from scRNAseq experiments performed on the epithelial compartment (Epcam+) of mouse adult sub-mandibular glands (C57BL/6J, female), either at baseline (wild type) or following stereotactic X-ray irradiation (30 Gy). In the specific case of wild-type mice, the dataset also includes data from a secind scRNAseq experiment performed on the basal compartment (Epcam+, Cd49f-high) of the sub-mandibular gland, which is postulated to contain a minority population of epithelial cells with stem-progenitor properties.

ORGANISM(S): Mus musculus

PROVIDER: GSE249106 | GEO | 2025/11/30

REPOSITORIES: GEO

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Project description:Although the salivary transcriptome of adult mosquitoes has been thoroughly described in several recent papers, very little information exists regarding the biological role of larval salivary glands in the Culicidae. We used whole-transcriptome AffymetrixÂ® chips to compare the transcriptional profiles of Anopheles gambiae larval (L4) salivary glands and whole larvae. We identified a total of 277 transcripts as being significantly enriched in the salivary glands. Based on available annotation for the known or predicted protein sequences encoded by these transcripts, 41 were identified as corresponding to secreted proteins, 233 as corresponding to non-secreted (housekeeping) proteins, and 3 as encoding proteins of unknown function. Based on functional annotation of the putatively secreted gene products, we propose that larval salivary secretions have roles in nutrient digestion, detoxification, immunity, and mouthpart lubrication. Interestingly, several components of the larval saliva (e.g. apyrase and serine proteases) have also been reported to exist in adult female saliva, where they are though to help regulate a vertebrate hostâs immune response to bloodfeeding. In conclusion, our results suggest that the salivary glands are important components of both the digestive and immune systems of larval mosquitoes, and that their study might provide clues about the evolution of adaptations to bloodfeeding observed in adults. Experiment Overall Design: We attempted to identify transcripts that are both present and significantly enriched in the salivary glands (SG) of the 4th instar Anopheles gambiae larva. Transcripts were considered as present in the SG if the built-in Affymetrix detection call scored them as such in all three biological replicates of the experiment. Expression values (i.e. signal intensities) of transcripts from the salivary glands were compared to those of a matching group of whole 4th instar larvae using a t-test. Transcripts showing an expression value two-fold or higher (p < 0.01; false discovery rate=1%) in the SG as compared to whole larvae were considered to be significantly enriched.

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Dissection by single-cell RNA-sequencing of the cellular composition of mouse salivary gland epithelia (wild-type and irradiated).

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