Transcriptomics

Dataset Information

30

Escherichia coli planktonic cultures: mono-species culture vs. mixed-species culture


ABSTRACT: Transcription profile of Escherichia coli cells in mono-species pure planktonic cultures was compared to that of E. coli cells in E. coli-Stenotrophomonas maltophilia dual-species planktonic cultures E. coli cells were separated from dual-species planktonic cultures before total RNA extraction to eliminate possible cross hybridization from S. maltophilia transcripts. The separation method was developed by combining the use of reagent RNAlater and immuno-magnetic separation. Pure E. coli planktonic cultures were processed with the same separation protocol before RNA extraction. Overall design: Two condition experiments: E. coli mono-species pure planktonic culture vs E. coli in mixed-species planktonic cultures. Two biological replicates with independently grown and harvested planktonic cultures. Each biological replicate has two technical replicates of hybridization on microarray slides. Each slide has three built-in replicates for each probe.

INSTRUMENT(S): University of Alberta Escherichia coli 19K v2.0

ORGANISM(S): Escherichia coli K-12  

SUBMITTER: Dongjuan Dai  

PROVIDER: GSE24913 | GEO |

SECONDARY ACCESSION(S): PRJNA133405

REPOSITORIES: GEO

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