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LXR agonism enhances T cell activation upon crosspresentation.

ABSTRACT: Here, we analyze transcriptional profiles of OT-I CD8+ T cells co-cultured with bone marrow-derived dendritic cells pulsed with the SIINFEKL peptide in the absence of presence of the synthetic LXR agonist RGX-104.

ORGANISM(S): Mus musculus

PROVIDER: GSE249709 | GEO | 2025/12/29

REPOSITORIES: GEO

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LXR agonism enhances T cell activation upon crosspresentation by LXR-DKO BMDCs.

Project description:Here, we analyze transcriptional profiles of OT-I CD8+ T cells co-cultured with LXR-/- bone marrow-derived dendritic cells pulsed with the SIINFEKL peptide in the absence or presence of the synthetic LXR agonist RGX-104.

2025-12-30 | GSE249710 | GEO

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LXR agonism elicits a pre-effector state in naïve CD8+ T cells [RNA-seq]

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LXR agonism elicits a pre-effector state in naïve CD8+ T cells [ATAC-seq]

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2025-12-29 | GSE303423 | GEO

scRNA-seq and TCR-seq of T cells and APCs in tumor-draining lymph nodes with versus without pharmacological LXR activation.

Project description:Here we assessed the impact on the transcriptomes of T cells and APCs in tumor-draining lymph nodes from mice administered control chow versus chow containing the synthetic LXR agonist RGX-104. In addition, TCR-seq was performed to assess the impact on clonal expansion of T cells upon LXR activation.

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Immunomodulatory-Photodynamic Nanostimulators for Invoking Pyroptosis to Augment Tumor Immunotherapy

Project description:Acidic tumor microenvironment (TME)-evoked MRC nanoparticles (MRC NPs) co-delivering immune agonist RGX-104 and photosensitizer chlorine e6 (Ce6) are reported for pyroptosis-mediated immunotherapy. RGX-104 remodels TME by transcriptional activation of ApoE to regress myeloid-derived suppressor cells’ (MDSCs) activity, which neatly creates foreshadowing for intensifying pyroptosis. Considering Ce6-triggered photodynamic therapy (PDT) can strengthen oxidative stress and organelles destruction to increase immunogenicity, immunomodulatory-photodynamic MRC nanodrugs will implement an aforementioned two-pronged strategy to enhance gasdermin E (GSDME)-dependent pyroptosis. RNA-seq analysis of MRC at the cellular level is introduced to first elucidate the intimate relationship between RGX-104 acting on LXR/ApoE axis and pyroptosis, where RGX-104 provides the prerequisite for pyroptosis participating in antitumor therapy. Briefly, MRC with favorable biocompatibility tackles the obstacle of hydrophobic drugs delivery, and becomes a powerful pyroptosis inducer to reinforce immune efficacy. MRC-elicited pyroptosis in combination with anti-PD-1 blockade therapy boosts immune response in solid tumors, successfully arresting invasive metastasis and extending survival based on remarkable antitumor immunity. MRC may initiate a new window for immuno-photo pyroptosis stimulators augmenting pyroptosis-based immunotherapy. Owing to the transcriptome sequencing on 4T1 cells exposed to various treatment, pyroptosis mechanism of MRC was thoroughly analyzed via comparing the expression discrepancy among PBS, MRC, and MRC+L groups.

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CD8 T cell transcriptional changes induced by MDSCs during TCR stimulation

Project description:Single cell RNA-seq analyiss of FACS enriched OT-I CD8+ T cells following co-culture with SIINFEKL-pulsed bone marrow-derived DCs and bone marrow-derived myeloid-derived suppressor cells (T cell:DC:MDSC = 1:0.2:2)

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In vivo screen identifies LXR agonism potentiates sorafenib killing of hepatocellular carcinoma

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2020-05-29 | GSE151412 | GEO

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