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MicroRNA-145 Regulates Human Corneal Epithelial Differentiation [Agilent-014850 array data]

ABSTRACT: To investigate the gene expression in human corneal epithelial overexpressing hsa-miR-145 by transfection , we have employed Whole Human Genome Oligo Microarray (Agilent) as a screening platform to identify gene regulation. We discovered a differential gene expression in HCE cells transfected with hsa-mIR-145 against cells with scrambled sequences. Among them, genes related with corneal development, integrity, differentiation and inflammatory responses were found and this was validated by real-time PCR.

ORGANISM(S): Homo sapiens

PROVIDER: GSE24980 | GEO | 2011/10/10

SECONDARY ACCESSION(S): PRJNA133463

REPOSITORIES: GEO

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MicroRNA-145 Regulates Human Corneal Epithelial Differentiation [Agilent-014850 array data]

Project description:To investigate the gene expression in human corneal epithelial overexpressing hsa-miR-145 by transfection , we have employed Whole Human Genome Oligo Microarray (Agilent) as a screening platform to identify gene regulation. We discovered a differential gene expression in HCE cells transfected with hsa-mIR-145 against cells with scrambled sequences. Among them, genes related with corneal development, integrity, differentiation and inflammatory responses were found and this was validated by real-time PCR. HCE cells transfected with hsa-miR-145 or scrambled sequences were collected at 24 hours after transfection. Total RNA was extracted by Trizol/chloroform and purified with RNeasy mini spin column. RNA samples with 28S/18S ratios in the range of 1.4 to 1.8 were used for expression RNA profiling using Whole Human Genome Oligo Microarray (Agilent).

2011-10-09 | E-GEOD-24980 | biostudies-arrayexpress

MicroRNA-145 Regulates Human Corneal Epithelial Differentiation [Agilent-016436 array data]

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Gene expression profiles of U87 cells after transfection with hsa-miR-145-5p and 31-5p

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MicroRNA-145 Regulates Human Corneal Epithelial Differentiation [Agilent-016436 array data]

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2011-10-09 | E-GEOD-24979 | biostudies-arrayexpress

MicroRNA-145 Regulates Human Corneal Epithelial Differentiation

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Project description:We have employed whole genome microarray to identify changes in gene expression in MDA-MB-231 cells transfected with hsa-miR-205-5p and hsa-miR-214-3p compared to scrambled miRNA precursors at 72 hours after transfection.

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miR-145 transfected PC3 cells

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