Transcriptome sequencing of jawbones and jawbone-derived mesenchymal stem cells from young and old mouse reveals the characteristics of aging jawbones

ABSTRACT: Jawbone mass gradually decreases during the aging process, resulting in tooth loosening or loss, impacting the oral chewing function, facial aesthetics, and quality of life of patients. Furthermore, it complicates restoration after tooth loss. However, there is a lack of research on transcriptional changes in the aging process of the jawbone. For this study, young mice aged 2 months and old mice aged 18 months were chosen as the research subjects. Jawbone tissues from both age groups were initially extracted and labeled as Y-JB (jawbones from young mice) and O-JB (jawbones from older mice). RNA-seq data of Y-JB and O-JB revealed 1321 up- and 1224 downregulated genes. The Gene Ontology (GO) analysis predominantly identified immune responses and protein binding, indicating their potential importance in jawbone senescence. Additionally, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis highlighted substantial differences in the PI3K/AKT signaling pathway and osteoclast differentiation in O-JB. The number of mesenchymal stem cells decreases with age, and these cells exhibit degenerative characteristics closely associated with the bone aging phenotype. Therefore, it is crucial to examine the aging phenotype in jawbone-derived mesenchymal stem cells (JBMSCs) during jawbone aging. RNA-seq of Young and aging JBMSCs revealed 1033 up- and 955 downregulated genes. GO analysis emphasized the regulation of the inflammatory response and ossification. Furthermore, KEGG pathway analysis indicated notable differences in the PI3K/AKT and mitogen-activated protein kinase signaling pathways in O-JBMSCs.

ORGANISM(S): Mus musculus

PROVIDER: GSE250121 | GEO | 2026/10/05

REPOSITORIES: GEO