Transcriptomics

Dataset Information

25

Expression profile of third instar larval fat body and midgut tissues


ABSTRACT: We use mRNA-seq to transcriptionally profile larval fat body and midgut tissues from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Overall design: Fat bodies from wandering third instar larvae were dissected from ~50 male larvae and gonads were removed to eliminate contaminating transctips from the gonads. Larval midguts were dissected from ~50 wandering third instar larvae. Larval tissues were removed to Graces unsupplemented medium on ice prior to RNA extraction with TRIzol reagent. mRNA-seq samples were prepared from 5ug of total RNA and subject to Illumina based sequencing.

INSTRUMENT(S): Illumina Genome Analyzer II (Drosophila melanogaster)

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Terry L. Orr-Weaver  

PROVIDER: GSE25025 | GEO | 2010-11-02

SECONDARY ACCESSION(S): PRJNA134399

REPOSITORIES: GEO

Similar Datasets

2010-11-02 | E-GEOD-25025 | ArrayExpress
2011-10-18 | GSE33017 | GEO
2011-10-18 | E-GEOD-33017 | ArrayExpress
2013-01-25 | E-GEOD-43734 | ArrayExpress
| GSE80475 | GEO
2007-10-31 | E-GEOD-3060 | ArrayExpress
2018-10-29 | E-MTAB-7195 | ArrayExpress
2013-02-27 | E-MTAB-699 | ArrayExpress
2018-09-27 | E-MTAB-7194 | ArrayExpress
2011-10-18 | E-GEOD-31899 | ArrayExpress