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Gene expression analysis of TRIM5 KD in the THP1 cell line

ABSTRACT: We had evidence that TRIM5 regulates signal transduction, specifically NFkB and MAPK pathways. To test the role of endogenous TRIM5 we used the myelomonocytic leukemia cell line THP1. These cells were transduced with a lentiviral vector that delivers a miRNA engineered to knockdown TRIM5. The vector also encoded a puromycin-resistance cassette and transduced cells were selected in poold with puromycin. As a control, cells were transduced with a vector targeting luciferase instead of TRIM5.

ORGANISM(S): Homo sapiens

PROVIDER: GSE25041 | GEO | 2010/11/02

SECONDARY ACCESSION(S): PRJNA134539

REPOSITORIES: GEO

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Gene expression analysis of TRIM5 KD in the THP1 cell line

Project description:We had evidence that TRIM5 regulates signal transduction, specifically NFkB and MAPK pathways. To test the role of endogenous TRIM5 we used the myelomonocytic leukemia cell line THP1. These cells were transduced with a lentiviral vector that delivers a miRNA engineered to knockdown TRIM5. The vector also encoded a puromycin-resistance cassette and transduced cells were selected in poold with puromycin. As a control, cells were transduced with a vector targeting luciferase instead of TRIM5. After selection in puromycin, the TRIM5 KD and Luciferase KD cells were differentiated into macrophages by treatment with PMA. 3 days later, RNA was harvested for analysis.

2010-11-02 | E-GEOD-25041 | biostudies-arrayexpress

ATAC-seq of Thp1-mono, Thp1-macro, and Thp1-M.tb

Project description:Next Generation Sequencing data of ATAC-Seq library of Thp1-mono, Thp1-macro, and Thp1-M.tb

2022-09-06 | GSE208044 | GEO

ChIP-seq of Thp1-mono, Thp1-macro, and Thp1-M.tb

Project description:Next Generation Sequencing data of ChIP-seq library of Thp1-mono, Thp1-macro, and Thp1-M.tb

2022-09-06 | GSE208043 | GEO

The effects of mutant ASXL1 and BAP1 expression on hematopoietic cells

Project description:To identify target genes of mutant ASXL1 and BAP1 in hematopoietic cells, we performed RNA-seq using murine c-kit positive cells transduced with ASXL1-MT (MT) or ASXL1-MT-K351R (KR) together with vector or BAP1. Method：Murine c-kit positive bone marrow cells were transduced with ASXL1-MT (MT) or ASXL1-MT-K351R (KR) (coexpressing blastcidin resistant gene) together with vector or BAP1 (coexpressing puromycin resistant gene). After the selection with blasticidin and puromycin for three days, colony-forming cells were collected to extract RNA for RNA-seq analysis.

2018-05-25 | GSE114861 | GEO

Impact of BHLHE40-AS1 over expression in MCF10A cells

Project description:BHLHE40-AS1 was cloned into pBABE-Puro. Empty vector and pBABE-BHLHE40-AS1 were used to generate retrovirus and infect MCF10A cells. Transduced cells were selected with 1ug/mL puromycin for 2 weeks.

2020-06-10 | GSE136579 | GEO

H3K4me2 and gene expression profiling in control and KMT2D KD murine B220 cells

Project description:Define and compare H3K4me2 enrichment in murine B220 cells transduced with empty vector (ct) or KMT2D-shRNA. Compare gene expression by RNAseq in murine B220 cells transduced with empty vector (ct) or KMT2D-shRNA.

2015-08-28 | GSE67291 | GEO

Expression data from THP1-wt and THP1-NTR+ cells with LSD1 inhibitor (OG86), Nitroreductase NfsB (NTR) prodrug and negative control

Project description:The Nitroreductase NfsB (NTR) prodrug of an LSD1 inhibitor is inactive in wild-type THP1 cells, but the prodrug gets activated in the LSD1 inhibitor by the NTR in NTR-expressing THP1 cells (THP1-NTR+). Cell-type specific inhibition of LSD1 in THP1-NTR+ cells with a NTR prodrug is shown with the expression data of the two cell lines, THP1-wt and THP1-NTR+. Data for the LSD1 inhibitor and a negative control with both cell lines are included.

2020-08-04 | GSE155640 | GEO

Chip-chip from THP1 cells with H3K56Ac

Project description:THP1 cells were ChIPed with H3K56Ac Comparison of selected chromatin marks in THP1 cells

2013-02-15 | E-GEOD-43868 | biostudies-arrayexpress

mRNA profiling of THP1 cell line

Project description:Analysis of mRNA in THP1 (human monocytic leukemia) cell line in order to correlate miRNA activity with target abundance. THP1 mRNA profiles were generated in triplicates by deep-sequencing in Illumina HiSeq2000.

2012-06-30 | E-GEOD-37769 | biostudies-arrayexpress

smallRNA profiling of THP1 cell line

Project description:Analysis of smallRNA in THP1 (human monocytic leukemia) cell line in order to correlate miRNA activity with target abundance. THP1 smallRNA profiles were generated in triplicates by deep-sequencing in Illumina HiSeq2000.

2012-06-30 | E-GEOD-37770 | biostudies-arrayexpress

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