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Generation of modified cattle and sheep from spermatid-like haploid stem cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Bos taurus Ovis aries

PROVIDER: GSE250497 | GEO | 2025/08/02

REPOSITORIES: GEO

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Generation of modified cattle and sheep from spermatid-like haploid stem cells

Project description:Generation of modified cattle and sheep from spermatid-like haploid stem cells

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Rapid production of modified cattle and sheep from spermatid-like stem cells [scRNA-seq]

Project description:Haploid androgenetic stem cells (haSCs) are invaluable resources for studying animal traits and greatly impact livestock breeding, but livestock haSCs have yet to be obtained. Here, we report the derivation of cattle and sheep haSCs using the recombined FACE medium, and these cells harbor formative pluripotency features. Notably, knockout cattle and sheep can be obtained upon injection of the protaminized haSCs with targeted gene mutations into intact oocytes.

2025-08-04 | GSE289354 | GEO

Rapid production of modified cattle and sheep from spermatid-like stem cells [CUT&Tag]

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Rapid production of modified cattle and sheep from spermatid-like stem cells [RNA-seq]

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Rapid generation of gene-edited cattle and sheep from spermatid-like formative haESCs [WGBS]

Project description:Haploid androgenetic embryonic stem cells (haESCs) can produce live mice, thus have potential for genetic manipulation. However, practical application is limited by self-diploidization, loss-of-imprint, low-birth-rate, and livestock haESCs have yet to be achieved. Here we show that combination of FACE medium supports establishment of durable haESCs from cattle and sheep that overcome above problems. Multi-omics and functional analysis of FACE-haESCs showed that at a naive-to-primed intermediate pre-gastrulation formative state of pluripotency. Moreover, transient protamine overexpression remodels ruminant haESCs nuclei into spermatid-like structures concomitant with removal of ICHI barrier, resulting blastocyst rate of Pro-ICHI similar to that of in vitro fertilization (IVF) embryos. As a proof-of-concept, we also developed a novel Pro-ICHI::ePE system to fast produce trait-improved cattle with exogene-free effects.

2025-08-04 | GSE250491 | GEO

Rapid generation of gene-edited cattle and sheep from spermatid-like formative haESCs [WGS]

2025-08-04 | GSE250496 | GEO

Rapid production of modified cattle and sheep from spermatid-like stem cells [CUT&Tag]

Project description:Rapid production of modified cattle and sheep from spermatid-like stem cells [CUT&Tag]

| PRJNA1222488 | ENA

Rapid production of modified cattle and sheep from spermatid-like stem cells [scRNA-seq]

Project description:Rapid production of modified cattle and sheep from spermatid-like stem cells [scRNA-seq]

| PRJNA1222558 | ENA

Rapid production of modified cattle and sheep from spermatid-like stem cells [RNA-seq]

Project description:Rapid production of modified cattle and sheep from spermatid-like stem cells [RNA-seq]

| PRJNA1222549 | ENA

Offspring production of haploid spermatid-like cells derived from mouse female germline stem cells with chromatin condensation.

Project description:BackgroundDuring male meiosis, the Y chromosome can form perfect pairing with the X chromosome. However, it is unclear whether mammalian Female germline stem cells (FGSCs) without a Y chromosome can transdifferentiate into functional haploid spermatid-like cells (SLCs).ResultsWe found that spermatogenesis was restarted by transplanting FGSCs into Kitw/wv mutant testes. Complete meiosis and formation of SLCs was induced in vitro by testicular cells of Kitw/wv mutant mice, cytokines and retinoic acid. Healthy offspring were produced by sperm and SLCs derived from the in vivo and in vitro transdifferentiation of FGSCs, respectively. Furthermore, high-throughput chromosome conformation capture sequencing(Hi-C-seq) and "bivalent" (H3K4me3-H3K27me3) micro chromatin immunoprecipitation sequencing (μChIP-seq) experiments showed that stimulated by retinoic acid gene 8 (STRA8)/protamine 1 (PRM1)-positive transdifferentiated germ cells (tGCs) and male germ cells (mGCs) display similar chromatin dynamics and chromatin condensation during in vitro spermatogenesis.ConclusionThis study demonstrates that sperm can be produced from FGSCs without a Y chromosome. This suggests a strategy for dairy cattle breeding to produce only female offspring with a high-quality genetic background.

| S-EPMC8729121 | biostudies-literature

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