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PcG/TrxG profiling of differentially aged adipose-derived mesenchymal stem cells

ABSTRACT: The microarray reported here is part of a study aimed to investigate whether the gene expression profile of Polycomb, Trithorax and interacting molecules (collectively referred to PcG/TrxG genes) might be related to different states of differentiative potential. In particular , this microarray profiles the expression of PcG/TrxG genes in adipose-derived mesenchymal stem cells isolated from the inguinal fat depot of mice of different age.

ORGANISM(S): Mus musculus

PROVIDER: GSE25068 | GEO | 2010/11/30

SECONDARY ACCESSION(S): PRJNA142733

REPOSITORIES: GEO

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PcG/TrxG profiling of differentially aged adipose-derived mesenchymal stem cells

Project description:The microarray reported here is part of a study aimed to investigate whether the gene expression profile of Polycomb, Trithorax and interacting molecules (collectively referred to PcG/TrxG genes) might be related to different states of differentiative potential. In particular , this microarray profiles the expression of PcG/TrxG genes in adipose-derived mesenchymal stem cells isolated from the inguinal fat depot of mice of different age. MSC have been isolated from the inguinal fat pad of FVB mice aged 1, 3, 6, 12 or 24 months.

2010-11-30 | E-GEOD-25068 | biostudies-arrayexpress

An essential role for PTIP in mediating Hox gene regulation along PcG and trxG pathways

Project description:During Drosophila development, Polycomb-group and Trithorax-group proteins function to ensure correct maintenance of transcription patterns by epigenetically repressing or activating target gene expression. To get a deep insight into the PcG and trxG pathways, we investigated a BRCT domain-containing protein called PTIP, which was generally identified as a transcriptional coactivator and belongs to the TRR complex. At the genome scale, we sorted given PTIP binding peaks into two groups: PTIP/TRR-cobound and PTIP/PC-cobound peaks. In particular, we found that PTIP mediates the molecular switch between H3K4me3/H3K27ac and H3K27me3 histone modifications at TRR or PC occupied regions. Thus, we suggest that PTIP is a mediator rather than a dedicated co-activator along PcG and trxG pathways. Our hypothesis is further supported by the genetic assay: PTIP interacts genetically with either PcG or TrxG in a dosage-dependent manner, suggesting that PTIP functions as a co-factor of PcG/TrxG proteins. In addition, in accordance with the analysis of ChIP-seq, these genetic interactions correlate with modified ectopic HOX protein levels in imaginal discs, which reveals an essential role for PTIP in PcG-mediated Hox gene repression. Hence, we reveal a novel role for PTIP in the epigenetic regulation of gene expression along PcG and trxG pathways.

2022-05-23 | GSE203601 | GEO

Chromatin immunopreciptiation to determine the genome wide distribution of PcG and TrxG proteins in Drosophila - part I

Project description:We used ChIP on chip assays to determine the genome wide distribution of a large set of PcG and trxG proteins, their associated histone marks and four candidate DNA-binding factors for PcG protein recruitment.

2008-07-26 | E-MEXP-1708 | biostudies-arrayexpress

Chromatin immunopreciptiation to determine the genome wide distribution of PcG and TrxG proteins in Drosophila - part II

2008-07-26 | E-MTAB-45 | biostudies-arrayexpress

Distribution of PcG, TrxG, RNA Pol II and associated histone marks in ML-DmD23-c4 cells

Project description:PcG and TrxG are important epigenetic regulators of genome expression. Here we have determined genomic distributions of PC, E(Z), H3K27me3, TRX, ASH1, RNA Pol II, H3K4me3, H3K27ac in cultured Drosophila ML-DmD23-c4 cells by hybridization of ChIP products with tiling microarrays

2010-01-08 | GSE18177 | GEO

Distribution of PcG, TrxG, RNA Pol II and associated histone marks in ML-DmBG3-c2 cells

Project description:PcG and TrxG are important epigenetic regulators of genome expression. Here we have determined genomic distributions of PC, E(Z), H3K27me3, TRX, ASH1, RNA Pol II, H3K4me3, H3K27ac in cultured Drosophila ML-DmBG3-c2 cells by hybridization of ChIP products with tiling microarrays.

2010-01-08 | GSE18100 | GEO

microRNA profiling of mesenchymal stem cells from adipose tissue of differentially aged mice

Project description:The data presented here is part of a study aimed to investigate whether the gene expression profile of Polycomb, Trithorax and interacting molecules might be related to different states of differentiative potential. microRNA profiling (Exiqon miRCURY LNA array Version 9.2) has been performed on mesenchymal stem cells isolated from the inguinal fat pad of mice of 1 (n=2), 3(n=2), 6(n=2), 12(n=2) and 24(n=1) months of age.

2010-11-30 | E-GEOD-25679 | biostudies-arrayexpress

A Polycomb Repressive Complex 1 proteome from Drosophila cells

Project description:Polycomb Group (PcG) proteins regulate gene expression through changes to chromatin structure and are essential for development. PcG proteins function together with the Trithorax Group (TrxG) proteins, which affect diverse steps in transcription activation. The PcG and TrxG are genetically and functionally antagonistic, and current understanding suggests a balance of their activities can maintain a wide range of transcription states. PcG and TrxG proteins assemble into a series of complexes. How these complexes work together, how PcG complexes are recruited to target genes, and whether other proteins are involved in their regulation of gene expression remain open questions. We carried out tandem affinity purification followed by mass spectrometry (TAP-MS) on two core components of Drosophila Polycomb Repressive Complex 1 (PRC1). Our data reveal a network of interactions among PcG complexes, and extensive co-purification of TrxG complexes with PRC1. We co-purified >50 transcription factors with PRC1 that were not previously linked to the PcG. Finally, we identify novel co-purifying complexes, including HP1c and PCNA handling complexes. Our resource of candidate PRC1 interacting proteins generate new hypotheses for PRC1 function.

2023-04-20 | MSV000091759 | MassIVE

Chromatin maps of the Drosophila melanogaster TrxG protein Ash1 and FSH

Project description:Trithorax group (TrxG) proteins counteract Polycomb silencing by an as yet uncharacterized mechanism. A well-known member of the TrxG is the histone methyltransferase Absent, Small, or Homeotic discs 1 (ASH1). In Drosophila ASH1 is needed for the maintenance of Hox gene expression throughout development, which is tightly coupled to preservation of cell identity. In order to understand the molecular function of ASH1 in this process, we performed affinity purification of tandem-tagged ASH1 followed by mass spectrometry (AP-MS) and identified FSH, another member of the TrxG as interaction partner. Here we provide genome-wide chromatin maps of both proteins based on ChIP-seq.

2013-06-30 | GSE30820 | GEO

Chromatin maps of the Drosophila melanogaster TrxG protein Ash1 and FSH

2013-06-30 | E-GEOD-30820 | biostudies-arrayexpress

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